Multi-omics analysis reveals the effects of three application modes of plant growth promoting microbes biofertilizer on potato (Solanum tuberosum L.) growth under alkaline loess conditions

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
Yuan Tian , Yang Liu , Liang Yue , Xia Zhao , Qin Zhou , Constantine Uwaremwe , Yun Wang , Gaofeng Chen , Yuexia Sha , Yubao Zhang , Ruoyu Wang
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Abstract

Potato is an important crop due to its high contents of starch, protein, and various vitamins and minerals. Biofertilizers are composed of plant growth promoting microbes (PGPMs) which are essential for improving the growth and resistance of potato. However, little information has focused on the modes of inoculation of biofertilizers on plant growth and microecology. This study aims to reveal the response mechanism of the potato to three modes of inoculation of biofertilizers all containing PGPM Bacillus amyloliquefaciens EZ99, i.e. scattered mode of 5 kg/ha biofertilizer (M5), soaking seed tubers with dissolved 5 kg/ha biofertilizer (MZG), and scattered mode of 3 kg/ha biofertilizer + 2 kg/ha sucrose (MY34) in alkaline loess field through multi-omics analysis of transcriptome, metabolome and microbiome. The physiological result revealed that two application modes of equal amount of biofertilizer M5 and MZG significantly improved the growth and yield of potatoes. Furthermore, the transcriptome of potato exhibited sets of differentially expressed genes enriched in photosynthesis, sugar metabolism, and phenylpropanoid biosynthesis among the three modes, with the M5 mode exhibiting overall up-regulation of 828 genes. Based on the untargeted metabolomic analysis of potato tuber, M5 mode significantly accumulated sucrose, while MZG and MY34 mode significantly accumulated the stress metabolites euchrenone b6 and mannobiose, respectively. Besides, the microbial structure of potato rhizosphere showed that the diversity of bacteria and fungi was similar in all soils, but their abundances varied significantly. Specifically, beneficial Penicillium was enriched in M5 and MZG soils, whereas MY34 soil accumulated potential pathogens Plectosphaerella and saccharophilic Mortierella. Collectively, these e findings highlight that MZG is the most effective mode to promote potato growth and stimulate rhizosphere effect. The present study not only encourages sustainable agriculture through agroecological practices, but also provides broad prospects for the application of PGPM biofertilizer in staple foods.

多组学分析揭示三种植物生长促进微生物生物肥料施用模式对碱性黄土条件下马铃薯(Solanum tuberosum L.)生长的影响
马铃薯是一种重要的农作物,因为它含有大量淀粉、蛋白质以及各种维生素和矿物质。生物肥料由植物生长促进微生物(PGPMs)组成,对改善马铃薯的生长和抗性至关重要。然而,有关接种生物肥料对植物生长和微生态的影响模式的信息却很少。本研究旨在揭示马铃薯对含有淀粉芽孢杆菌 EZ99 的三种生物肥料接种模式的反应机制,即通过对转录组、代谢组和微生物组的多组学分析,发现在碱性黄土地上,马铃薯对三种接种生物肥料(均含有 PGPM 直链芽孢杆菌 EZ99)的模式,即每公顷 5 千克生物肥料的分散接种模式(M5)、用溶解的每公顷 5 千克生物肥料浸泡块茎种子的接种模式(MZG)和每公顷 3 千克生物肥料+每公顷 2 千克蔗糖的分散接种模式(MY34)。生理结果表明,等量施用生物肥 M5 和 MZG 可显著提高马铃薯的生长和产量。此外,马铃薯的转录组显示,三种施肥模式在光合作用、糖代谢和苯丙类生物合成方面富集了多组差异表达基因,其中 M5 模式总体上调了 828 个基因。根据马铃薯块茎的非靶向代谢组学分析,M5模式显著积累蔗糖,而MZG和MY34模式分别显著积累胁迫代谢产物优克龙b6和甘露寡糖。此外,马铃薯根瘤菌层的微生物结构表明,所有土壤中细菌和真菌的多样性相似,但丰度差异很大。具体而言,M5 和 MZG 土壤中富含有益的青霉,而 MY34 土壤中则富含潜在的病原菌 Plectosphaerella 和嗜糖 Mortierella。总之,这些发现突出表明,MZG 是促进马铃薯生长和刺激根瘤效应的最有效模式。本研究不仅鼓励通过农业生态实践实现农业的可持续发展,还为 PGPM 生物肥料在主食中的应用提供了广阔的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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