Acceleration of wound healing using adipose mesenchymal stem cell secretome hydrogel on partial-thickness cutaneous thermal burn wounds: An in vivo study in rats

IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Suryo Kuncorojakti, Awidhan Zainal Adi Pratama, Cahya Asri Antujala, Clarence Theodosius Bernard Harijanto, Rozak Kurnia Arsy, Putut Andika Kurniawan, Yudy Tjahjono, Lucia Hendriati, Teguh Widodo, Ahmad Aswin, D. Diyantoro, Andi Yasmin Wijaya, Watchareewan Rodprasert, Helen Susilowati
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Abstract

Background and Aim: The intricate healing process involves distinct sequential and overlapping phases in thermal injury. To maintain the zone of stasis in Jackson’s burn wound model, proper wound intervention is essential. The extent of research on the histoarchitecture of thermal wound healing and the application of mesenchymal stem cell (MSC)-free-based therapy is limited. This study aimed to assess the efficacy of MSC-secretome-based hydrogel for treating partial-thickness cutaneous thermal burn wounds. Materials and Methods: Eighteen male Wistar rats were divided into three groups, namely the hydrogel base (10 mg), hydrogel secretome (10 mg) and Bioplacenton™ (10 mg) treatment groups. All groups were treated twice a day (morning and evening) for 7 days. Skin tissue samples from the animals were processed for histological evaluation using the formalin-fixed paraffin-embedded method on days 3 and 7. Results: This study’s findings showed that secretome hydrogel expedited thermal burn wound healing, decreasing residual burn area, boosting collagen deposition and angiogenesis, guiding scar formation, and influencing the inflammation response facilitated by polymorphonuclear leukocytes and macrophages. Conclusion: The secretome hydrogel significantly improves healing outcomes in partial-thickness cutaneous thermal burn wounds. The administration of secretome hydrogel accelerates the reduction of the residual burn area and promotes fibroblast proliferation and collagen density. The repairment of histo-architecture of the damaged tissue was also observed such as the reduction of burn depth, increased angiogenesis and epidermal scar index while the decreased dermal scar index. Furthermore, the secretome hydrogel can modulate the immunocompetent cells by decreasing the polymorphonuclear and increasing the mononuclear cells. Thus, it effectively and safely substitutes for thermal injury stem cell-free therapeutic approaches. The study focuses on the microscopical evaluation of secretome hydrogel; further research to investigate at the molecular level may be useful in predicting the beneficial effect of secretome hydrogel in accelerating wound healing. Keywords: drug safety, mesenchymal stem cell-conditioned media, stem cell-free-therapy, thermal injury, wound healing.
使用脂肪间充质干细胞分泌物水凝胶加速部分厚度皮肤热烧伤创面的愈合:大鼠体内研究
背景和目的:在热损伤中,错综复杂的愈合过程涉及不同的顺序和重叠阶段。要维持杰克逊烧伤模型中的停滞区,适当的伤口干预至关重要。有关热损伤愈合的组织结构和无间充质干细胞疗法应用的研究十分有限。本研究旨在评估基于间充质干细胞分泌物的水凝胶治疗部分厚度皮肤热烧伤创面的疗效。材料与方法:将 18 只雄性 Wistar 大鼠分为三组,即水凝胶基质组(10 毫克)、水凝胶分泌物组(10 毫克)和 Bioplacenton™ 组(10 毫克)。所有组每天治疗两次(早晚各一次),共治疗 7 天。在第 3 天和第 7 天,采用福尔马林固定石蜡包埋法对动物的皮肤组织样本进行组织学评估。研究结果研究结果表明,泌素体水凝胶可加速热烧伤伤口愈合,减少烧伤残留面积,促进胶原沉积和血管生成,引导疤痕形成,并影响多形核白细胞和巨噬细胞促进的炎症反应。结论分泌物水凝胶能明显改善部分厚度皮肤热烧伤创面的愈合效果。服用分泌物水凝胶可加快烧伤残留面积的缩小,促进成纤维细胞增殖和胶原蛋白密度。同时还观察到受损组织的组织结构得到修复,如烧伤深度减少、血管生成增加、表皮疤痕指数增加、真皮疤痕指数减少。此外,分泌物水凝胶还能调节免疫细胞,减少多形核细胞,增加单核细胞。因此,它能有效、安全地替代不含热损伤干细胞的治疗方法。本研究侧重于对分泌物水凝胶的显微评估;进一步研究分子水平的调查可能有助于预测分泌物水凝胶在加速伤口愈合方面的有益效果。关键词:药物安全性、间充质干细胞调节介质、无干细胞疗法、热损伤、伤口愈合。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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