Mechanism of inactivation of Pseudomonas fluorescens by plasma-activated water based on active substances

IF 6.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Innovative Food Science & Emerging Technologies Pub Date : 2024-08-01 DOI:10.1016/j.ifset.2024.103764

Duanying Huang , Qinchao Sun , Jiaojiao Meng , Jie Xu , Yanmo Pan , Ouyang Zheng , Shucheng Liu , Qinxiu Sun

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引用次数: 0

Abstract

The physicochemical properties of plasma-activated water (PAW) and the mechanism of PAW on the inactivation of Pseudomonas fluorescens were investigated. The results demonstrated that as activation time and output voltage increased, the electrical conductivity (EC) and oxidation-reduction potential (ORP) of PAW increased, while its pH decreased. When the activation condition was 20 kV for 30 min (PAW30), the content of active substances of the PAW was the highest (P < 0.05). The concentrations of NO₂⁻, NO₃⁻, and H₂O₂ were high as 0.43 μg/mL, 2.46 μg/mL, and 0.837 μg/mL, respectively. After the PAW30 treatment for 30 min, the population of P. fluorescens was decreased by 2.27 log₁₀ CFU/mL when the initial inoculation volume was 4.38 log₁₀ CFU/mL. PAW oxidative stress disrupted the balance of the intracellular redox, ion concentration, and pH, leading to the breakdown of cell wall and membrane integrity, ultimately causing bacterial inactivation. These are important mechanisms by which PAW exerts its bactericidal effect.

Industrial relevance

The aim of this study was to clarify the optimal preparation conditions for PAW, and its mechanism of killing P. fluorescens. These results contribute to our understanding of the control of spoilage organisms in aquatic products by PAW. In the raw material treatment stage of aquatic product processing, PAW can be used to clean and soak raw materials to kill microorganisms on their surfaces of raw materials. Therefore, PAW can be applied as a green cleaning technology to improve the hygienic quality of aquatic products.

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基于活性物质的等离子活化水灭活荧光假单胞菌的机制

研究了等离子体活化水（PAW）的理化性质及其对荧光假单胞菌的灭活机理。结果表明，随着活化时间和输出电压的增加，等离子活化水的电导率（EC）和氧化还原电位（ORP）升高，pH值降低。当活化条件为 20 kV 30 min（PAW30）时，PAW 的活性物质含量最高（P < 0.05）。NO2-、NO3-和H2O2的浓度分别高达0.43 μg/mL、2.46 μg/mL和0.837 μg/mL。PAW30 处理 30 分钟后，当初始接种量为 4.38 log10 CFU/mL 时，P. fluorescens 的数量减少了 2.27 log10 CFU/mL。PAW 氧化应激破坏了细胞内氧化还原、离子浓度和 pH 值的平衡，导致细胞壁和细胞膜完整性遭到破坏，最终导致细菌失活。本研究旨在阐明 PAW 的最佳制备条件及其杀灭荧光团菌的机制。这些结果有助于我们理解 PAW 对水产品中腐败微生物的控制作用。在水产品加工的原料处理阶段，PAW 可用于清洗和浸泡原料，杀灭原料表面的微生物。因此，PAW 可作为一种绿色清洁技术用于提高水产品的卫生质量。

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来源期刊

Innovative Food Science & Emerging Technologies 工程技术-食品科技

CiteScore

12.00

自引率

6.10%

发文量

259

审稿时长

25 days

期刊介绍： Innovative Food Science and Emerging Technologies (IFSET) aims to provide the highest quality original contributions and few, mainly upon invitation, reviews on and highly innovative developments in food science and emerging food process technologies. The significance of the results either for the science community or for industrial R&D groups must be specified. Papers submitted must be of highest scientific quality and only those advancing current scientific knowledge and understanding or with technical relevance will be considered.