The Performance of the Current Algorithm of HIV Diagnosis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-13 DOI:10.18502/ijph.v53i7.16051

Abdurrahman Gulmez, Ozgur Appak, A. N. Zeka, Nuran Esen, A. Sayıner

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引用次数: 0

Abstract

Background: We aimed to evaluate the performance of the current algorithm the HIV diagnosis that has been performed for four years. Results of HIV Ag/Ab tests, anti-HIV 1/2 confirmatory tests, HIV-1 RNA tests and the time for official results to be approved were evaluated. Methods: The relationship of HIV Ag/Ab tests, anti-HIV 1/2 confirmation tests and HIV-1 RNA PCR tests, their result times and suitability to the algorithm were examined at Izmir Dokuz Eylül University between May 2017 and June 2021. Results: HIV Ag/Ab ELISA was reactive repetitively in 165/54628 (0.30%) serum samples. Anti-HIV 1/2 confir-mation test was reactive in 54.42% (80/147) of samples. The most common pattern (18.2%) in the con-firmation tests was the positivity of the antibodies against gp160 - gp41 envelope glycoproteins together. The mean reporting time of the confirmation test result was 3h 50 min after the ELISA test. The mean reporting time of the HIV-1 RNA PCR was 12.79 d (±10.22) after the ELISA test and 12.63 (± 10.12) day after the confirmation test. In ROC analysis, the estimated rate of the ELISA test for the confirmation test was highest when S/CO was >13.16 (sensitivity: 97.59 %, specificity: 97.59%). Conclusion: The confirmation test in the current algorithm enabled the rapid test results, early diagnosis of HIV and early antiretroviral therapy. To use the new algorithm effectively, decentralization of the validation tests would be appropriate.

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当前艾滋病毒诊断算法的性能

背景：我们的目的是评估已实施四年的现行艾滋病诊断算法的性能。我们对 HIV Ag/Ab 检测结果、抗 HIV 1/2 确证检测结果、HIV-1 RNA 检测结果以及正式结果获得批准的时间进行了评估。方法在 2017 年 5 月至 2021 年 6 月期间，在伊兹密尔 Dokuz Eylül 大学对 HIV Ag/Ab 检测、抗 HIV 1/2 确认检测和 HIV-1 RNA PCR 检测的关系、结果时间和算法的适用性进行了研究。结果显示在 165/54628 份（0.30%）血清样本中，HIV Ag/Ab ELISA 呈重复反应。54.42% （80/147）的样本中，抗 HIV 1/2confir-mation检测呈反应性。确证试验中最常见的模式（18.2%）是 gp160 - gp41 包膜糖蛋白抗体同时阳性。确认检测结果的平均报告时间为 ELISA 检测后 3 小时 50 分钟。HIV-1 RNA PCR 的平均报告时间为 ELISA 检测后 12.79 天（±10.22）和确认检测后 12.63 天（±10.12）。在 ROC 分析中，当 S/CO >13.16 时，ELISA 检测用于确认检测的估计比率最高（灵敏度：97.59%，特异性：97.59%）。结论现行算法中的确证试验能够快速得出检测结果，及早诊断出艾滋病病毒，并及早进行抗逆转录病毒治疗。为有效使用新算法，应分散进行确认检验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.