Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 in banana plantations in Türkiye

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2024-07-17 DOI:10.36253/phyto-15133

M. Özarslandan, D. S. Akgül

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Abstract

important disease for banana production. Presence and prevalence of FocTR4 in banana plantations on the Mediterranean coast of Türkiye were assessed during 2018 to 2020 in a total of 117 banana plantations in open fields and protected plastic greenhouses. Rhizome, pseudostem, and root samples were taken from plants showing typical symptoms associated to the disease and from suspected affected plants. Fungi were isolated from the plant internal tissues, and Fusarium oxysporum-like colonies were sub-cultured for further analyses. Phylogenetic analyses of 36 isolates showed that they belonged to four different Fusarium species: F. musae, F. oxysporum, F. sacchari, and F. solani. Eight representative F. oxysporum isolates were identified as FocTR4 by specific PCR and qPCR tests. Pathogenicity tests were carried out on tissue-cultured ‘Cavendish’ type banana seedlings (‘Grand Naine’) for 36 Fusarium isolates, and their virulence was assessed based on the internal necrosis observed in the rhizomes. Approx. 40 to 65 d after inoculations, FocTR4, F. oxysporum, and F. sacchari isolates caused severe to mild necroses in the seedling rhizomes. This is the first report of F. sacchari associated with root and collar rot of bananas in Türkiye. This study showed that Fusarium wilt caused by FocTR4 is present, but at low incidence (6.8%) in Turkish banana plantations.

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图尔基耶香蕉种植园中由 Fusarium oxysporum f. sp. cubense tropical race 4 引起的镰刀菌枯萎病

是香蕉生产的重要病害。2018 年至 2020 年期间，在土耳其地中海沿岸的露天田地和受保护的塑料大棚中，对总共 117 个香蕉种植园中 FocTR4 的存在和流行情况进行了评估。从表现出与该疾病相关的典型症状的植株和疑似受影响的植株中采集根茎、假茎和根样本。从植物内部组织中分离出真菌，并对类似氧孢镰刀菌的菌落进行亚培养，以便进一步分析。对 36 个分离菌株的系统发育分析表明，它们属于四个不同的镰刀菌种：F. musae、F. oxysporum、F. sacchari 和 F. solani。通过特异性 PCR 和 qPCR 检测，8 个具有代表性的 F. oxysporum 分离物被鉴定为 FocTR4。在组织培养的 "卡文迪许 "型香蕉幼苗（"Grand Naine"）上对 36 个镰刀菌分离物进行了致病性试验，并根据根茎中观察到的内部坏死情况评估了它们的毒力。接种后约 40 到 65 d，FocTR4、F. oxysporum 和 F. sacchari 分离物导致幼苗根茎出现严重到轻度坏死。这是土耳其首次报道 F. sacchari 与香蕉根腐病和领腐病有关。这项研究表明，土耳其香蕉种植园中存在由 FocTR4 引起的镰刀菌枯萎病，但发病率较低（6.8%）。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.