The Effect of Aqueous Extract of Fenugreek Plant on the Pancreas in Rabbits Induced with Diabetes and the Histological Changes Occurring in Them

Abstract

The study aimed to induce alloxan-induced diabetes in local white rabbits, study its effect on the pancreas, treat them with aqueous extract of fenugreek at a concentration of 200 mg/kg orally, and observe the histological changes. To complete the study 20 white rabbits were taken and divided into three groups; 1st group is the control group and this group was dosed with distilled water. 2nd group was treated with Alloxan and was injected intraperitoneally at a dose of 175 mg/kg. 3rd group is the group that was treated with the aqueous extract of fenugreek at a concentration of 200 mg/kg for 30 days. After the end of the injection and dosing period after 30 days, the animals were killed and the pancreas was removed to perform histological techniques on them. its the group treated with alloxan was observed that the pancreas was composed of exocrine glandular lobules with enlarged secretory cells within the pancreatic acinus, with the presence of pale nuclei lacking pigment. Also, blood congestion of the blood vessels was found between the lobules of the gland and the pancreatic gland showed hyperplasia of its glandular epithelial cells with nuclei devoid of pigment due to the loss of the basal pigment in them. Blood congestion of the blood vessels appeared in the gland between the lobules, and dense colloidal fibers appeared in them and contained fibroblasts and phagocytic cells and the group treated with the aqueous extract of the fenugreek plant :The pancreatic parenchyma contained normal exocrine glandular secretion units, where the cytoplasm and nuclei of these cells were located in the form of regular axes, septations between the lobules of the pancreas surrounded by thick-walled arteries containing blood. and the islets of Lankerhans appeared between the yeast-secreting lobules of the pancreas. They contained endocrine cells of alpha and beta cells surrounded by blood sinusoids. The interstitial tissue between the lobules is composed of loose connective tissue containing colloidal fibers the pancreatic tissue contained the serous secretory vasculature of yeasts, which were uniform in shape, and between them were found blood vessels excessive with blood, where blood was found in a decomposed form in them, with the presence of small channels inside the lobules that transport yeasts.