Is there any difference in Lactobacillus abundance between age groups during ovarian stimulation in fresh in vitro fertilization (IVF) cycles?

Q2 Medicine

Medicinski Glasnik Pub Date : 2024-07-23 DOI:10.17392/1715-21-02

Haris Zukić, Devleta Balić, Džanan Osmanović

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引用次数: 0

Abstract

Aim To investigate an influence of age and body mass index (BMI) as determinants of infertility treatment success in vitro fertilization (IVF) procedures confirmed so far, on the amount of Lactobacillus spp. (LS) and Gardnerella vaginalis (GV) in the endometrium in fresh IVF cycles at the time of embryo transfer.
Methods This a prospective single-centre study included patients who underwent fresh embryo transfer (ET) in a private IVF Centre. A catheter tip for embryo transfer was used for collecting samples from the endometrial cavity. Real time polymerase chain reaction (RT PCR) was used to determine LS and GV quantity. Patients were divided into two age groups: <35 years and ≥35 years. Normal BMI group included patients with 18.-24.9 kg/m², and the in-creased BMI group patients with BMI ≥ 25kg/m².
Results Tips for embryo transfer catheters were positive on LS in 55 (96.4%) patients. The median of quantification cycle of RT-PCR (Cq RT-PCR) for LS was 33, and 31 for the younger and older groups, respectively. Twenty-one (36.8%) patients tested positive for GV. The median of Cq RT-PCR for GV was 33 and 34 for the younger and older groups, respectively. There were 36 patients with normal and 18 with increased BMI. BMI showed no correlation with LS amount in the endometrial cavity.
Conclusion Aging affects the endome-trial microbiome resulting in an increase of Lactobacillus number, but not the number of GV.

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在新鲜体外受精（IVF）周期的卵巢刺激过程中，不同年龄组的乳酸杆菌丰度是否存在差异？

目的研究年龄和体重指数（BMI）作为目前已证实的不孕症治疗成功率的决定因素，对胚胎移植时新鲜试管婴儿周期子宫内膜中乳酸杆菌（LS）和阴道加德纳菌（GV）数量的影响。使用胚胎移植导管从子宫内膜腔采集样本。采用实时聚合酶链反应（RT PCR）测定 LS 和 GV 的数量。患者被分为两个年龄组：35 岁组和 35 岁组。结果 55 例（96.4%）患者的胚胎移植导管尖端在 LS 上呈阳性。年轻组和年长组 LS 的 RT-PCR 定量周期（Cq RT-PCR）中位数分别为 33 和 31。21名患者（36.8%）的GV检测呈阳性。年轻组和年长组 GV RT-PCR 的 Cq 中位数分别为 33 和 34。36 名患者的体重指数正常，18 名患者的体重指数升高。结论老龄化会影响子宫内膜-子宫腔微生物组，导致乳酸杆菌数量增加，但不会增加 GV 的数量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medicinski Glasnik 医学-医学：内科

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Medicinski Glasnik (MG) is the official publication (two times per year) of the Medical Association of Zenica-Doboj Canton. Manuscripts that present of original basic and applied research from all fields of medicine (general and clinical practice, and basic medical sciences) are invited.