Assessment of Solvent Extraction using Sonication to Recover Tryptophan from Kappaphycus alvarezii (Doty) Doty ex Silva: Experimental and Modelling

Trends in Sciences Pub Date : 2024-07-24 DOI:10.48048/tis.2024.8197

Venansius G. P. Putra, Weni Chaniago, Nuzulia Izmi, Selma Mutiarahma, Kiki Adi Kurnia, Miguel Palma, W. Setyaningsih

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Abstract

The application of Kappaphycus alvarezii in the food system has attracted researchers due to its bioactive compounds, including tryptophan. The present study was conducted to extract tryptophan from K. alvarezii with the aid of sonication. A reduced multilevel factorial design was conducted to evaluate the effect of solvent, ultrasound power, duty-pulse cycle, time and temperature on the recovery of tryptophan. Analysis of variance suggested that the type of solvent and combination setting of power and duty-pulse cycle significantly influenced the extraction (p < 0.05). In contrast, extraction time and temperature did not alter the extraction (p > 0.05). The optimum was using ethanol, ultrasound power 80 %, pulse duty-cycle 0.8 s–1, extraction time 10 min, and temperature 25 °C and tryptophan concentration was 56.41 ± 2.42 mg L–1. Subsequently, a COnducto-like Screening MOdel for Real Solvent (COSMO-RS) was performed to clarify the impact of solvent affinity and polarity on the tryptophan extraction from K. alvarezii.

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利用超声波进行溶剂萃取以从 Kappaphycus alvarezii (Doty) Doty ex Silva 中回收色氨酸的评估：实验与建模

由于卡帕蕨（Kappaphycus alvarezii）具有包括色氨酸在内的生物活性化合物，其在食品系统中的应用吸引了众多研究人员。本研究利用超声技术从 K. alvarezii 中提取色氨酸。研究采用简化多级因子设计，评估了溶剂、超声功率、占空比、时间和温度对色氨酸回收率的影响。方差分析表明，溶剂类型以及功率和占空比的组合设置对提取有显著影响（p < 0.05）。相反，萃取时间和温度对萃取没有影响（p > 0.05）。最佳提取条件为乙醇、超声功率 80 %、脉冲占空比 0.8 s-1、提取时间 10 分钟、温度 25 °C，色氨酸浓度为 56.41 ± 2.42 mg L-1。随后，为了明确溶剂亲和性和极性对从阿尔瓦雷斯藻类中提取色氨酸的影响，进行了类似于真实溶剂的 COnducto-like Screening MOdel（COSMO-RS）试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Sciences

CiteScore

1.20

自引率

0.00%

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