Differences in tissue-Level Properties as Assessed by Nano-Scratching in Patients With and Without Atypical Femur Fractures on Long-Term Bisphosphonate Therapy: A Proof-of-Concept Pilot Study

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Gabriella Johnson, Lanny V Griffin, Shijing Qiu, S. Rao
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Abstract

Atypical femur fractures (AFF) are a well-established complication of long-term bisphosphonate (BP) therapy, but their pathogenesis is not fully understood. Although many patients on long-term BP therapy have severe suppression of bone turnover (SSBT), not all such patients AFF even though SSBT is a major contributor to AFF. Accordingly, we evaluated tissue level properties using nano-scratch testing of trans-iliac bone biopsy specimens in 12 women (6 with and 6 without AFF matched for age and race). Nano-scratch data were analyzed using a mixed model ANOVA with volume normalized scratch energy as a function of AFF (Yes or No), region (periosteal or endosteal), and a first-order interaction between region and AFF. Tukey Post-hoc analyses of the differences of least squared means of scratch energy were performed and reported as significant if P<.05. The volume normalized scratch energy was 10.6% higher in AFF than in non-AFF patients (P=.003) and 17.9 % higher in the periosteal than in the endosteal region (P=.004). The differences in normalized scratch energy are consistent with a higher hardness of the bone tissue after long-term BP therapy. The results of this study are consistent with other studies in the literature and demonstrate the efficacy of using Nano-Scratch technique to evaluate bone tissue that exhibits SSBT and AFF. Further studies using nano-scratch may help quantify and elucidate underlying mechanisms for the pathogenesis of AFF.
通过纳米划痕评估长期接受双膦酸盐治疗的非典型股骨骨折患者和非典型股骨骨折患者的组织级特性差异:概念验证试点研究
非典型股骨骨折(AFF)是长期双膦酸盐(BP)疗法的一种公认并发症,但其发病机制尚未完全明了。尽管许多长期接受双膦酸盐治疗的患者骨转换(SSBT)受到严重抑制,但并非所有此类患者都会发生非典型股骨骨折,尽管 SSBT 是导致非典型股骨骨折的主要因素。因此,我们使用纳米划痕测试评估了 12 名女性(6 名有 AFF,6 名没有 AFF,年龄和种族匹配)经髂骨活检标本的组织水平特性。纳米划痕数据采用混合模型方差分析,将体积归一化划痕能量作为 AFF(是或否)、区域(骨膜或骨膜内)以及区域和 AFF 之间一阶交互作用的函数。对划痕能最小平方均值的差异进行 Tukey 后差异分析,如果 P<.05 则认为差异显著。AFF患者的体积归一化划痕能比非AFF患者高10.6%(P=.003),骨膜区比骨内区高17.9%(P=.004)。归一化划痕能的差异与长期 BP 治疗后骨组织硬度增加一致。本研究的结果与其他文献中的研究结果一致,证明了使用纳米划痕技术评估表现出 SSBT 和 AFF 的骨组织的有效性。使用纳米划痕技术进行的进一步研究可能有助于量化和阐明 AFF 的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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