Electron microscopy study of left ventricular cardiomyocytes in adult rats born preterm

IF 1.5 0 LANGUAGE & LINGUISTICS

Morphology Pub Date : 2024-07-27 DOI:10.17816/morph.631920

V. Ivanova

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引用次数: 0

Abstract

BACKGROUND: Preterm birth is a risk factor for the early development of cardiovascular diseases. To date, based on the results of clinical studies, it is impossible to get a notion of the ultrastructural features of cardiomyocytes in adolescents and adults born prematurely. In this regard, it is relevant to conduct an experiment aimed at studying the effect of preterm birth on the ultrastructure of cardiomyocytes in the late postnatal period of ontogenesis. AIM: to study the ultrastructure of left ventricular cardiomyocytes in 24-hour premature rats on the 180th day of the postnatal period of ontogenesis. METHODS: The study was conducted on full-term (n=4, pregnancy duration 22 days) and preterm (n=4, pregnancy duration 21 days) male Wistar rats. Preterm labor was induced by mifepristone injection to pregnant rats. Preterm and full-term offspring were removed from the experiment on the 180th day of the postnatal period of ontogenesis. Fragments of the left ventricle of the heart of preterm and full-term rats were used for ultrastructural studies of cardiomyocytes (transmission electron microscopy). Electron microphotographs of longitudinal sections of contractile cardiomyocytes used to determination of the relative areas of the nucleus, cytoplasm, myofibrils, and mitochondria. RESULTS: The structure of cardiomyocytes of preterm and full-term rats on the 180th day of the postnatal period is fundamentally similar. However, the relative area of the nuclei of cardiomyocytes in preterm rats is lower, and the relative area of the cytoplasm is higher than in full-term animals. Exclusively in the cytoplasm of preterm rats, perinuclear swelling of the cytoplasm, thinning of myofibrils, as well as signs of mitochondrial damage or dysfunction, such as destruction of mitochondrial membranes, concentric organization of mitochondrial cristae, dissociation of mitochondrial clusters, are observed. CONCLUSION: Preterm birth has long-lasting effects on cardiomyocyte ultrastructure. The observed ultrastructural changes indicate a disturbance in energy production in the cardiomyocytes of preterm rats in the late postnatal period of ontogenesis.

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早产成年大鼠左心室心肌细胞的电子显微镜研究

背景：早产是心血管疾病早期发展的一个危险因素。迄今为止，根据临床研究的结果，还无法了解早产青少年和成人心肌细胞的超微结构特征。因此，有必要进行一项实验，研究早产对出生后晚期本体发育阶段心肌细胞超微结构的影响。目的：研究出生后第 180 天的 24 小时早产大鼠左心室心肌细胞的超微结构。方法：研究对象为足月（n=4，妊娠期 22 天）和早产（n=4，妊娠期 21 天）雄性 Wistar 大鼠。向怀孕大鼠注射米非司酮诱发早产。早产和足月后代在出生后第180天从实验中取出。早产大鼠和足月大鼠的左心室片段被用于心肌细胞的超微结构研究（透射电子显微镜）。通过对收缩心肌细胞纵切面的电子显微照，确定细胞核、细胞质、肌纤维和线粒体的相对面积。结果：早产大鼠和足月大鼠在出生后第 180 天的心肌细胞结构基本相似。但是，早产大鼠心肌细胞核的相对面积低于足月大鼠，而胞质的相对面积高于足月大鼠。只有在早产大鼠的细胞质中，才能观察到细胞质核周肿胀、肌纤维变细以及线粒体损伤或功能障碍的迹象，如线粒体膜破坏、线粒体嵴同心组织、线粒体簇解离等。结论：早产会对心肌细胞超微结构产生长期影响。观察到的超微结构变化表明，早产大鼠的心肌细胞在产后晚期的本体发育过程中能量生产出现了紊乱。

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来源期刊

Morphology LANGUAGE & LINGUISTICS-

CiteScore

3.60

自引率

0.00%

发文量

期刊介绍： Aim The aim of Morphology is to publish high quality articles that contribute to the further articulation of morphological theory and linguistic theory in general, or present new and unexplored data. Relevant empirical evidence for the theoretical claims in the articles will be provided by in-depth analyses of specific languages or by comparative, cross-linguistic analyses of the relevant facts. The sources of data can be grammatical descriptions, corpora of data concerning language use and other naturalistic data, and experiments. Scope Morphology publishes articles on morphology proper, as well as articles on the interaction of morphology with phonology, syntax, and semantics, the acquisition and processing of morphological information, the nature of the mental lexicon, and morphological variation and change. Its main focus is on formal models of morphological knowledge, morphological typology (the range and limits of variation in natural languages), the position of morphology in the architecture of the human language faculty, and the evolution and change of language. In addition, the journal deals with the acquisition of morphological knowledge and its role in language processing. Articles on computational morphology and neurolinguistic approaches to morphology are also welcome. The first volume of Morphology appeared as Volume 16 (2006). Previous volumes were published under the title Yearbook of Morphology.