Network pharmacology and in vitro experiments reveal the autophagy mechanism of Yanggan-Yishui granules in improving hypertensive renal injury

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Pan Liu, Tairan Hu, Bing Gao, Ran Xia, Xiaohua Dai, Jing Wang
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引用次数: 0

Abstract

In China, Yanggan-Yishui granules (YGYSG) have been used to treat hypertensive renal damage (HRD) for over 20 years. Network pharmacology was used to determine whether YGYSG affects HRD via the autophagy pathway, which was verified using in vitro experiments.Common targets of YGYSG, HRD, and the autophagy pathway were screened using network pharmacology, and effective compounds, core targets, and signaling pathways were identified. The affinity of the compounds for the core targets was evaluated using molecular docking simulations. Angiotensin II (Ang II) was used to generate an in vitro renal podocyte model using MPC-5 cells. Morphological changes in the autophagosomes were observed using transmission electron microscopy (TEM). The expression levels of autophagy-related and pathway proteins were detected using western blotting and reverse transcription quantitative real-time PCR (PCR).Network pharmacology and molecular docking analyses identified eight autophagy-related core targets and ten core components in the YGYSG treatment of HRD. These targets are mainly involved in the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway and autophagy-related biological processes. In vitro experiments showed that Ang II-stimulated renal podocytes exhibited abnormal autophagy, and YGYSG protected renal podocytes from abnormal autophagy. In addition, YGYSG reversed abnormal autophagy and improved HRD by activating the PI3K/AKT/mTOR signaling pathway.YGYSG may regulate abnormal autophagy in renal podocytes by activating the PI3K/AKT/mTOR signaling pathway and may play a role in improving HRD.
网络药理学和体外实验揭示养干益水颗粒改善高血压肾损伤的自噬机制
在中国,阳甘益水颗粒(YGYSG)用于治疗高血压肾损害(HRD)已有20多年的历史。利用网络药理学确定了YGYSG是否通过自噬途径影响HRD,并通过体外实验进行了验证。利用网络药理学筛选了YGYSG、HRD和自噬途径的共同靶点,并确定了有效化合物、核心靶点和信号通路。利用分子对接模拟评估了化合物与核心靶点的亲和力。利用血管紧张素 II(Ang II)和 MPC-5 细胞生成体外肾脏荚膜细胞模型。利用透射电子显微镜(TEM)观察了自噬体的形态变化。网络药理学和分子对接分析确定了YGYSG治疗HRD的8个自噬相关核心靶点和10个核心组分。这些靶点主要参与磷酸肌醇3-激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶标(mTOR)信号通路和自噬相关的生物过程。体外实验表明,Ang II 刺激的肾脏荚膜细胞表现出异常的自噬,而 YGYSG 能保护肾脏荚膜细胞免受异常自噬的影响。此外,YGYSG 还能通过激活 PI3K/AKT/mTOR 信号通路逆转异常自噬并改善 HRD。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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