A bilayer bioengineered patch with sequential dual-growth factor release to promote vascularization in bladder reconstruction.

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-07-25 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae083
Jian Zhao, Haoqian Zhang, Zhengyun Ling, Ziyan An, Shuwei Xiao, Pengchao Wang, Zhouyang Fu, Jinpeng Shao, Yanfeng Sun, Weijun Fu
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引用次数: 0

Abstract

Bladder tissue engineering holds promise for addressing bladder defects resulting from congenital or acquired bladder diseases. However, inadequate vascularization significantly impacts the survival and function of engineered tissues after transplantation. Herein, a novel bilayer silk fibroin (BSF) scaffold was fabricated with the capability of vascular endothelial growth factor (VEGF) and platelet derived growth factor-BB (PDGF-BB) sequential release. The outer layer of the scaffold was composed of compact SF film with waterproofness to mimic the serosa of the bladder. The inner layer was constructed of porous SF matrix incorporated with SF microspheres (MS) loaded with VEGF and PDGF-BB. We found that the 5% (w/v) MS-incorporated scaffold exhibited a rapid release of VEGF, whereas the 0.2% (w/v) MS-incorporated scaffold demonstrated a slow and sustained release of PDGF-BB. The BSF scaffold exhibited good biocompatibility and promoted endothelial cell migration, tube formation and enhanced endothelial differentiation of adipose derived stem cells (ADSCs) in vitro. The BSF patch was constructed by seeding ADSCs on the BSF scaffold. After in vivo transplantation, not only could the BSF patch facilitate the regeneration of urothelium and smooth muscle, but more importantly, stimulate the regeneration of blood vessels. This study demonstrated that the BSF patch exhibited excellent vascularization capability in bladder reconstruction and offered a viable functional bioengineered patch for future clinical studies.

双层生物工程贴片可连续释放双重生长因子,促进膀胱重建中的血管化。
膀胱组织工程有望解决先天性或后天性膀胱疾病导致的膀胱缺陷问题。然而,血管化不足会严重影响移植后工程组织的存活和功能。在本文中,我们制作了一种新型双层丝纤维素(BSF)支架,它能依次释放血管内皮生长因子(VEGF)和血小板衍生生长因子-BB(PDGF-BB)。支架的外层由具有防水功能的紧凑型 SF 薄膜组成,以模拟膀胱的浆膜。内层由多孔 SF 基质构成,基质中含有载入血管内皮生长因子和 PDGF-BB 的 SF 微球(MS)。我们发现,含 5%(w/v)MS 的支架能快速释放血管内皮生长因子,而含 0.2%(w/v)MS 的支架则能缓慢、持续地释放 PDGF-BB。BSF 支架具有良好的生物相容性,在体外可促进内皮细胞迁移、管形成并增强脂肪衍生干细胞(ADSCs)的内皮分化。通过在 BSF 支架上播种 ADSCs,构建了 BSF 补丁。体内移植后,BSF补片不仅能促进尿路上皮和平滑肌的再生,更重要的是能刺激血管的再生。这项研究表明,BSF补片在膀胱重建中表现出卓越的血管化能力,为未来的临床研究提供了一种可行的功能性生物工程补片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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