Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus.

IF 4.3 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Tingting Zhang, Shenjie Ji, Miaomiao Zhang, Fei Wu, Xue Li, Xi Luo, Qinglian Huang, Min Li, Yiquan Zhang, Renfei Lu
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Abstract

Vibrio vulnificus, a significant marine pathogen, undergoes opaque (Op)-translucent (Tr) colony switching based on whether capsular polysaccharide (CPS) is produced. CPS phase variation is sometime accompanied by genetic variation or down-regulation of particular genes, such as wzb. In addition, CPS prevents biofilm formation and is important to the virulence of V. vulnificus. However, the extent to which there is a difference in gene expression between Tr and Op colonies and the impact of CPS phase variation on other behaviors of V. vulnificus remain unknown. In this work, the data have shown that CPS phase variation of V. vulnificus is affected by incubation time. Tr and Op strains exhibited similar growth rates. However, Tr strains had enhanced biofilm formation capacities but reduced swimming motility compared to Op strains. The RNA-seq assay revealed 488 differentially expressed genes, with 214 downregulated and 274 upregulated genes, between Tr and Op colonies. Genes associated with Tad pili and CPS were downregulated, whereas those involved in flagellum were upregulated, in Tr colonies compared with Op colonies. In addition, 9 putative c-di-GMP metabolism-associated genes and 28 genes encoding putative regulators were significantly differentially expressed, suggesting that CPS phase variation is probably strictly regulated in V. vulnificus. Moreover, 8 genes encoding putative porins were also differentially expressed between the two phenotypic colonies, indicating that bacterial outer membrane was remodeled during CPS phase variation. In brief, this work highlighted the gene expression profiles associated with CPS phase variation, but more studies should be performed to disclose the intrinsic mechanisms in the future.

胶囊多糖相位变化对弧菌生物膜形成、运动和基因表达的影响
弧菌是一种重要的海洋病原体,会根据是否产生荚膜多糖(CPS)进行不透明(Op)-半透明(Tr)菌落切换。CPS 的阶段性变化有时伴随着基因变异或特定基因(如 wzb)的下调。此外,CPS 可防止生物膜的形成,对弧菌的毒力非常重要。然而,Tr菌落和Op菌落之间基因表达的差异程度以及CPS阶段性变化对V. vulnificus其他行为的影响仍然未知。在这项工作中,数据显示弧菌的 CPS 相位变化受孵育时间的影响。Tr菌株和Op菌株的生长速度相似。然而,与 Op 菌株相比,Tr 菌株的生物膜形成能力更强,但游动能力却有所下降。RNA-seq检测发现,Tr菌株和Op菌株有488个基因表达不同,其中214个基因下调,274个基因上调。与 Op 菌株相比,Tr 菌株中与 Tad pili 和 CPS 相关的基因下调,而与鞭毛相关的基因上调。此外,9个与c-di-GMP代谢相关的推定基因和28个编码推定调节因子的基因有显著差异表达,这表明弧菌的CPS相位变化可能受到严格调控。此外,8 个编码假定孔蛋白的基因在两个表型菌落之间也有差异表达,表明细菌外膜在 CPS 相变过程中发生了重塑。简而言之,这项工作强调了与 CPS 阶段变异相关的基因表达谱,但未来应开展更多研究以揭示其内在机制。
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来源期刊
Gut Pathogens
Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY
CiteScore
7.70
自引率
2.40%
发文量
43
期刊介绍: Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).
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