Effect and Mechanism of 6-Gingerol against Precancerous Lesions of Gastric Cancer Based on Network Pharmacology and In Vitro Experiment.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2024-07-29 DOI:10.2174/0113862073303380240722112207

Jingyu Xu, Cian Zhang, Ling Wang, Meiling Wang, JIayu Liang, Yanlong Yang, Xuan Zhang

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引用次数: 0

Abstract

Background: Precancerous Lesions of Gastric Cancer (PLGC) are critical in the secondary prevention of gastric cancer. Despite the notable effects of natural products on PLGC, the specific mechanisms by which compounds, like 6-gingerol, influence these lesions are not fully understood.

Aims: This study aimed to confirm the effect and mechanism of 6-gingerol in the treatment of precancerous lesions of gastric cancer (PLGC).

Objective: The objective of this study was to elucidate the effects and mechanisms of 6-gingerol against PLGC using network pharmacology and in vitro experiments.

Methods: We employed network pharmacology to identify potential targets and pathways influenced by 6-gingerol, followed by validation through in vitro experiments using a PLGC cell model.

Results: Network pharmacology analysis highlighted the PI3K/Akt signaling pathway as significantly influenced by 6-gingerol. In vitro experiments confirmed that 6-gingerol effectively inhibited proliferation, invasion, and metastasis of MC cells, promoted apoptosis, and induced cell cycle arrest, primarily through modulation of the PI3K/Akt pathway. Statistical analysis revealed significant inhibition (p < 0.05) across these cellular processes in a dose-dependent manner.

Conclusion: This study demonstrated that 6-gingerol acts as an effective agent against PLGC, with clear dose-dependent effects that pave the way for further experimental and clinical exploration.

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基于网络药理学和体外实验的 6-姜酚对胃癌癌前病变的作用和机制

背景：胃癌癌前病变（PLGC）是胃癌二级预防的关键。尽管天然产物对胃癌前病变有显著效果，但人们对 6-姜酚等化合物影响胃癌前病变的具体机制还不完全了解：本研究旨在利用网络药理学和体外实验阐明6-姜酚对胃癌癌前病变的作用和机制：方法：我们利用网络药理学来确定受6-姜酚影响的潜在靶点和通路，然后利用PLGC细胞模型通过体外实验进行验证：结果：网络药理学分析显示，PI3K/Akt 信号通路受到 6-gingerol 的显著影响。体外实验证实，6-姜酚主要通过调节 PI3K/Akt 通路，有效抑制 MC 细胞的增殖、侵袭和转移，促进细胞凋亡，诱导细胞周期停滞。统计分析显示，6-姜黄素以剂量依赖的方式对这些细胞过程有明显的抑制作用（p < 0.05）：本研究表明，6-姜酚是一种有效的抗肿瘤药物，具有明显的剂量依赖性，为进一步的实验和临床探索铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.