Effects of Photobiomodulation Associated with Platelet-Rich Plasma in Acute Rheumatoid Arthritis Induced in Female Wistar Rats' Knee.

IF 1.8 Q2 SURGERY
Bruna Silva Gomes, Amanda Bezerra Gonçalves, Sabrina Zanchetta Lanza, Marcelo Augusto Marretto Esquisatto, Fernando Russo Costa do Bomfim, Gaspar de Jesus Lopes Filho
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Abstract

Objective: Rheumatoid arthritis causes inflammation, pain, and joint degradation, necessitating treatment with anti-inflammatory drugs and corticosteroids, posing various challenges. We aimed to evaluate the effects of photobiomodulation (PBM) at two different doses associated to platelet-rich plasma (PRP) in an in vivo model of induced acute arthritis in Wistar rats' knee. Methods: Eighty-four Wistar rats were assigned into seven groups, including animals treated with PBM and/or PRP. On day 0, arthritis was induced in sham and treated groups through the intra-articular injection of zymosan (200 μg). Twenty-four hours after induction, the PBM groups were treated with an AsGaAl laser, whereas the PRP-treated groups received intra-articular injections with a concentration of 8 × 105 platelets obtained from another four animals. After 3 days, the animals were euthanized, and the interleukin (IL)-6 and complement C3 gene and protein expression levels were analyzed. Statistical analysis was performed using the mean ± SD with analysis of variance and Tukey's posttest, with a significance level set at 5% (p < 0.05). Results: Synovial inflammation decreased in PBM-treated groups; however, PRP alone showed no significant difference. Gene expression analysis revealed a significant difference in IL-6 and C3 levels in the PBM and PBM+PRP-treated groups. Meanwhile, the PRP alone group exhibited significance for IL-6. Moreover, the PBM and PBM+PRP-treated groups showed a significant difference in C3 protein expression levels, whereas the PRP alone group showed no difference. Conclusion: The increase in cellular activity in the synovial membrane and the decrease protein expression levels are owing to the reduction in proinflammatory mediators following PBM therapy.

光生物调节与富血小板血浆对雌性 Wistar 大鼠膝关节急性类风湿性关节炎的影响
目的:类风湿性关节炎会导致炎症、疼痛和关节退化,因此必须使用抗炎药物和皮质类固醇进行治疗,这带来了各种挑战。我们的目的是评估两种不同剂量的光生物调节(PBM)与富血小板血浆(PRP)在 Wistar 大鼠膝关节急性关节炎体内诱导模型中的效果。研究方法将 84 只 Wistar 大鼠分为 7 组,其中包括接受 PBM 和/或 PRP 治疗的动物。第 0 天,通过关节内注射齐莫散(200 μg)诱发假组和治疗组的关节炎。诱导24小时后,PBM组接受AsGaAl激光治疗,而PRP治疗组则在关节内注射从另外四只动物身上获得的浓度为8 × 105的血小板。3 天后,动物安乐死,并分析白细胞介素(IL)-6 和补体 C3 基因及蛋白表达水平。统计分析采用均数±标准差、方差分析和 Tukey 后验法,显著性水平设定为 5%(P < 0.05)。结果PBM治疗组的滑膜炎症有所减轻;但单用PRP治疗组的差异不大。基因表达分析表明,PBM 和 PBM+PRP 治疗组的 IL-6 和 C3 水平有显著差异。同时,单用 PRP 组的 IL-6 也有显著差异。此外,PBM 和 PBM+PRP 治疗组的 C3 蛋白表达水平有显著差异,而单纯 PRP 组则无差异。结论滑膜细胞活性的增加和蛋白表达水平的降低是由于 PBM 治疗后促炎介质的减少。
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来源期刊
CiteScore
4.10
自引率
0.00%
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期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
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