Antifungal recombinant psoriasin of human origin effectively inhibits fungal growth on denture base.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-07-28 DOI:10.1111/odi.15092

Lucia Adriana Lifshits, Edward Bronshtein, May Attias, Yoav Breuer, Adi Cohen, Matan Gabay, Marina Sova, Evgeny Weinberg, Eran Zenziper, Daniel Z Bar, Nir Sterer, Maayan Gal

{"title":"Antifungal recombinant psoriasin of human origin effectively inhibits fungal growth on denture base.","authors":"Lucia Adriana Lifshits, Edward Bronshtein, May Attias, Yoav Breuer, Adi Cohen, Matan Gabay, Marina Sova, Evgeny Weinberg, Eran Zenziper, Daniel Z Bar, Nir Sterer, Maayan Gal","doi":"10.1111/odi.15092","DOIUrl":null,"url":null,"abstract":"Objective: To evaluate the efficacy of recombinant psoriasin as a novel treatment for oral candidiasis by eliminating Candida albicans growth on polymethyl methacrylate denture base.Materials and methods: Recombinant psoriasin protein was expressed and purified from E. coli, and Candida growth was monitored in vitro with varying concentrations of psoriasin. Subsequently, denture-base polymethyl methacrylate was immersed in psoriasin's solution or voriconazole, and fungal growth on the acrylic base and in the medium was examined by scanning electron microscopy and optical density, respectively. Cellular viability of HeLa and human gingival fibroblast cells treated with psoriasin was measured by methylene blue assay.Results: The findings reveal an effective antifungal activity of psoriasin, completely inhibiting Candida albicans growth in RPMI at a protein concentration above 400 nM. Immersing the polymethyl methacrylate with 50 μM psoriasin completely eradicates fungal growth. Psoriasin has low cytotoxicity in HeLa cells at a concentration higher than 12 μM and no toxic effect on human gingival fibroblasts.Conclusions: This study marks psoriasin as an effective alternative to conventional antifungal treatments for denture stomatitis and a safe alternative to chemical antifungals in dental medicine and beyond.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"672-681"},"PeriodicalIF":2.9000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.15092","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/28 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To evaluate the efficacy of recombinant psoriasin as a novel treatment for oral candidiasis by eliminating Candida albicans growth on polymethyl methacrylate denture base.

Materials and methods: Recombinant psoriasin protein was expressed and purified from E. coli, and Candida growth was monitored in vitro with varying concentrations of psoriasin. Subsequently, denture-base polymethyl methacrylate was immersed in psoriasin's solution or voriconazole, and fungal growth on the acrylic base and in the medium was examined by scanning electron microscopy and optical density, respectively. Cellular viability of HeLa and human gingival fibroblast cells treated with psoriasin was measured by methylene blue assay.

Results: The findings reveal an effective antifungal activity of psoriasin, completely inhibiting Candida albicans growth in RPMI at a protein concentration above 400 nM. Immersing the polymethyl methacrylate with 50 μM psoriasin completely eradicates fungal growth. Psoriasin has low cytotoxicity in HeLa cells at a concentration higher than 12 μM and no toxic effect on human gingival fibroblasts.

Conclusions: This study marks psoriasin as an effective alternative to conventional antifungal treatments for denture stomatitis and a safe alternative to chemical antifungals in dental medicine and beyond.

查看原文本刊更多论文

源于人类的抗真菌重组牛皮癣素能有效抑制义齿基托上的真菌生长。

目的通过消除白色念珠菌在聚甲基丙烯酸甲酯义齿基托上的生长，评估重组银屑病素作为口腔念珠菌病新型疗法的疗效：从大肠杆菌中表达和纯化重组银屑病素蛋白，用不同浓度的银屑病素在体外监测念珠菌的生长。随后，将义齿基底聚甲基丙烯酸甲酯浸入银屑病素溶液或伏立康唑中，分别用扫描电子显微镜和光密度法检测丙烯酸基底和培养基中的真菌生长情况。用亚甲基蓝测定法测量了经银屑病素处理的 HeLa 细胞和人牙龈成纤维细胞的细胞活力：结果：研究结果表明，银屑病素具有有效的抗真菌活性，当蛋白浓度超过 400 nM 时，它能完全抑制白色念珠菌在 RPMI 中的生长。用 50 μM 银翘散浸泡聚甲基丙烯酸甲酯可完全根除真菌生长。当浓度高于 12 μM 时，银屑病素对 HeLa 细胞的细胞毒性较低，对人类牙龈成纤维细胞无毒性作用：这项研究表明，Psoriasin 是治疗义齿口腔炎的传统抗真菌疗法的有效替代品，也是牙科医学及其他领域化学抗真菌药物的安全替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.