A transcriptomic analysis of incisional hernia based on high-throughput sequencing technology.

IF 2.6 2区医学 Q1 SURGERY

Hernia Pub Date : 2024-10-01 Epub Date: 2024-07-29 DOI:10.1007/s10029-024-03116-7

Yiming Lin, Hekai Shi, Rongduo Yang, Shaochun Li, Jianxiong Tang, Shaojie Li

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引用次数: 0

Abstract

Purpose: Incisional hernia is a common postoperative complication; however, few transcriptomic studies have been conducted on it. In this study, we used second-generation high-throughput sequencing to explore the pathogenesis and potential therapeutic targets of incisional hernias.

Methods: Superficial fasciae were collected from 15 patients without hernia and 21 patients with an incisional hernia. High-throughput sequencing of the fascia was performed to generate an expression matrix. We analyzed the matrix to identify differentially expressed genes (DEGs) and performed gene ontology and enrichment analyses of these DEGs. Additionally, an external dataset was utilized to identify key DEGs.

Results: We identified 1,823 DEGs closely associated with extracellular matrix (ECM) imbalance, bacterial inflammatory response, and fibrillar collagen trimerization. TNNT3, CMAY5, ATP1B4, ASB5, CILP, SIX4, FBN1 and FNDC5 were identified as key DEGs at the intersection of the two expression matrices. Moreover, non-alcoholic fatty liver disease-related, TNF, and IL-17 signaling pathways were identified as key enrichment pathways.

Conclusions: We identified eight key DEGs and three pathways associated with incisional hernias. Our findings offer new insights into the pathogenesis of incisional hernias and highlight potential targets for their prevention and treatment.

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基于高通量测序技术的切口疝转录组分析。

目的：切口疝是一种常见的术后并发症，但有关它的转录组学研究却很少。在这项研究中，我们利用第二代高通量测序技术探索切口疝的发病机制和潜在治疗靶点：方法：从 15 名无疝气患者和 21 名切口疝患者身上采集浅筋膜。对筋膜进行高通量测序，生成表达矩阵。我们对矩阵进行了分析，以确定差异表达基因（DEGs），并对这些 DEGs 进行了基因本体和富集分析。此外，我们还利用外部数据集来确定关键的 DEGs：结果：我们发现了 1823 个与细胞外基质（ECM）失衡、细菌炎症反应和纤维胶原三聚化密切相关的 DEGs。TNNT3、CMAY5、ATP1B4、ASB5、CILP、SIX4、FBN1和FNDC5被确定为两种表达矩阵交叉处的关键DEGs。此外，非酒精性脂肪肝相关、TNF和IL-17信号通路也被确定为关键富集通路：结论：我们发现了与切口疝相关的八个关键 DEGs 和三个通路。结论：我们发现了与切口疝相关的八个关键 DEGs 和三个通路。我们的发现为切口疝的发病机制提供了新的见解，并突出了预防和治疗切口疝的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hernia SURGERY-

CiteScore

4.90

自引率

26.10%

发文量

171

审稿时长

4-8 weeks

期刊介绍： Hernia was founded in 1997 by Jean P. Chevrel with the purpose of promoting clinical studies and basic research as they apply to groin hernias and the abdominal wall . Since that time, a true revolution in the field of hernia studies has transformed the field from a ”simple” disease to one that is very specialized. While the majority of surgeries for primary inguinal and abdominal wall hernia are performed in hospitals worldwide, complex situations such as multi recurrences, complications, abdominal wall reconstructions and others are being studied and treated in specialist centers. As a result, major institutions and societies are creating specific parameters and criteria to better address the complexities of hernia surgery. Hernia is a journal written by surgeons who have made abdominal wall surgery their specific field of interest, but we will consider publishing content from any surgeon who wishes to improve the science of this field. The Journal aims to ensure that hernia surgery is safer and easier for surgeons as well as patients, and provides a forum to all surgeons in the exchange of new ideas, results, and important research that is the basis of professional activity.