Genomic Insights of a Methicillin-Resistant Biofilm-Producing Staphylococcus aureus Strain Isolated From Food Handlers.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-07-20 eCollection Date: 2024-01-01 DOI:10.1155/2024/5516117
Fatimah Muhammad Ballah, M Nazmul Hoque, Md Saiful Islam, Golam Mahbub Faisal, Al-Muksit Mohammad Taufiquer Rahman, Mst Minara Khatun, Marzia Rahman, Jayedul Hassan, Md Tanvir Rahman
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引用次数: 0

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm-producing and multidrug-resistant (MDR) S. aureus MTR_BAU_H1 strain through whole-genome sequencing (WGS). The S. aureus MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the S. aureus MTR_BAU_H1 strain is a strong biofilm-former and carries both antimicrobial resistance (e.g., methicillin resistance; mecA, beta-lactam resistance; blaZ and tetracycline resistance; tetC) and virulence (e.g., sea, tsst, and PVL) genes. The genome of the S. aureus MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first-ever WGS-based study of a strong biofilm-producing and MDR S. aureus strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.

从食品处理人员中分离出的耐甲氧西林生物膜金黄色葡萄球菌菌株的基因组研究。
耐甲氧西林金黄色葡萄球菌(MRSA)是一种重要的人畜共患病原体,与人类和动物的多种感染有关。因此,MRSA 克隆的出现对人类和动物健康构成了重要威胁。本研究旨在通过全基因组测序(WGS)阐明一株强生物膜产生型耐多药(MDR)金黄色葡萄球菌 MTR_BAU_H1 的基因组学特征。金黄色葡萄球菌 MTR_BAU_H1 株是从孟加拉国食品从业人员的手拭子中分离出来的,并进行了抗菌药敏感性和生物膜产生测定的表型评估。分离菌株进一步进行了高通量 WGS 检测,并使用不同的生物信息学工具进行分析,以阐明其遗传多样性、分子流行病学、序列类型(ST)、抗菌药耐药性和毒力基因分布。表型分析表明,金黄色葡萄球菌 MTR_BAU_H1 菌株具有很强的生物膜形成能力,同时携带抗菌素耐药性(如甲氧西林耐药性;mecA、β-内酰胺耐药性;blaZ 和四环素耐药性;tetC)和毒力基因(如 sea、tsst 和 PVL)。金黄色葡萄球菌 MTR_BAU_H1 的基因组属于 ST1930,拥有 3 个质粒复制子(如 rep16、rep7c 和 rep19)、7 个噬菌体和 2 个大小不等的簇状规则间隔短回文重复(CRISPR)阵列。系统进化分析表明,MTR_BAU_H1基因组与其他不同宿主和人群的MRSA克隆之间存在密切的进化关系。MTR_BAU_H1 基因组包含 42 个抗菌药耐药基因 (ARG)、128 个毒力基因和 273 个 SEED 子系统,编码氨基酸、碳水化合物、蛋白质、辅助因子、维生素、矿物质和脂质的代谢。这是首次基于 WGS 对从孟加拉国人体手拭子中分离出的强生物膜产生和 MDR 金黄色葡萄球菌菌株进行的研究,揭示了可能与人和动物的葡萄球菌致病机制有关的抗性基因组(ARGs 和相关机制)和毒力潜能的新信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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