Oxylipin profiling analyses reveal that ω-3 PUFA is more susceptible to lipid oxidation in sheep testis under oxidative stress

IF 2.2 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Wanhong Li , Ting Yao , Xinyue Zhang , Xiuxiu Weng , Fadi Li , Xiangpeng Yue
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Abstract

Reactive oxygen species causes oxidative stress, which oxidizes polyunsaturated fatty acids (PUFAs) to form oxidative metabolites. Sertoli cell is an important cellular metabolism of PUFA in testicular cells, and it regulates the testis development and spermatogenesis. However, the oxylipins generated in testes with different developmental statuses are lacking. In this study, twelve 6-month-old Hu sheep were selected and divided into large testicular group (L) and the small testicular group (S) (n=6). UPLC–MS/MS was conducted to screen oxylipins in the testis, and the total oxylipin and ω-3 PUFA-derived oxylipin contents in the S group were higher. A total of 20 differential oxylipins between the two groups were screened. Among them, the contents of ω-3 PUFA, DHA-derived oxylipins were increased in the S group. The arachidonic acid-derived oxylipin was lower in the S group. The mRNA expression levels of genes related to oxylipin regulation (AKR1B1, PTGER2, and PTGDS) were higher in the S group (P < 0.05). In vitro, 200 µM α-linolenic acid alleviated oxidative stress damage to Sertoli cells and improved cell viability by increasing the superoxide dismutase contents and mRNA expression levels of GPX4 and Bcl2. These results indicate that ω-3 PUFA is more susceptible to lipid oxidation in the S group under oxidative stress, which might alleviate the damage of oxidative stress to testis. Moreover, ALA could stimulate the proliferation of Sertoli cells by increasing the capacity of antioxidants. This work may provide a theoretical basis for further studies on the antioxidant properties of the testis for Hu sheep.

氧化脂分析表明,ω-3 PUFA 在绵羊睾丸氧化压力下更容易被脂质氧化。
活性氧会导致氧化应激,氧化多不饱和脂肪酸(PUFA)形成氧化代谢产物。Sertoli细胞是睾丸细胞中重要的多不饱和脂肪酸代谢细胞,它调控着睾丸的发育和精子的生成。然而,目前还缺乏不同发育状态的睾丸所产生的氧脂蛋白。本研究选择了 12 只 6 个月大的胡羊,将其分为大睾丸组(L)和小睾丸组(S)(n=6)。通过UPLC-MS/MS对睾丸中的草脂素进行筛选,发现S组的总草脂素和ω-3 PUFA衍生草脂素含量较高。两组共筛选出20种不同的草脂素。其中,S 组的ω-3 PUFA、DHA 衍生的氧脂素含量增加。S组花生四烯酸衍生的氧脂素含量较低。与氧脂调节相关的基因(AKR1B1、PTGER2 和 PTGDS)的 mRNA 表达水平在 S 组中较高(P < 0.05)。在体外,200 µM α-亚麻酸通过增加超氧化物歧化酶的含量以及 GPX4 和 Bcl2 的 mRNA 表达水平,减轻了氧化应激对 Sertoli 细胞的损伤,并提高了细胞的活力。这些结果表明,ω-3 PUFA 在氧化应激下更易被 S 组脂质氧化,从而可能减轻氧化应激对睾丸的损伤。此外,ALA还能通过提高抗氧化剂的能力来刺激Sertoli细胞的增殖。这项研究可为进一步研究胡羊睾丸的抗氧化特性提供理论依据。
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来源期刊
Animal Reproduction Science
Animal Reproduction Science 农林科学-奶制品与动物科学
CiteScore
4.50
自引率
9.10%
发文量
136
审稿时长
54 days
期刊介绍: Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.
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