DNA nanosensor based on bipedal 3D DNA walker–driven proximal catalytic hairpin assembly for sensitive and fast TK1 mRNA detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-07-29 DOI:10.1007/s00604-024-06569-w

Hang Gong, Shufen Yao, Xiaojia Zhao, Feng Chen, Chunyan Chen, Changqun Cai

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引用次数: 0

Abstract

Hyperproliferative diseases are the first step for tumor formation; thymidine kinase 1 (TK1) mRNA is closely related to cell proliferation. Therefore, the risk of malignant proliferation can be identified by sensitively detecting the variance in TK1 mRNA concentration, which can be used for tumor auxiliary diagnosis and monitoring tumor treatment. Owing to the low abundance and instability of TK1 mRNA in real samples, the development of a sensitive and fast mRNA detection method is necessary. A DNA nanosensor that can be used for detecting TK1 mRNA based on bipedal 3D DNA walker–driven proximal catalytic hairpin assembly (P-CHA) was developed. P-CHA hairpins were hybridized to a linker DNA strand coupled with magnetic nanoparticles to increase their local concentrations. The bipedal DNA walking on the surface of NPs accelerates reaction kinetics using the proximity effect. Taking advantage of the signal amplification of P-CHA as well as the rapid reaction rate of the DNA walker in 80 min, the proposed sensor detects TK1 mRNA with a low detection limit of 14 pM and may then be applied to clinical diagnosis.

Graphical Abstract

Abstract Image

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基于双足三维 DNA 步行器驱动的近端催化发夹组装的 DNA 纳米传感器，用于灵敏快速地检测 TK1 mRNA。

过度增殖性疾病是肿瘤形成的第一步；胸苷激酶 1（TK1）mRNA 与细胞增殖密切相关。因此，可以通过灵敏检测 TK1 mRNA 浓度的变化来识别恶性增殖的风险，从而用于肿瘤辅助诊断和监测肿瘤治疗。由于真实样本中 TK1 mRNA 的丰度低且不稳定，因此有必要开发一种灵敏快速的 mRNA 检测方法。基于双足三维 DNA 步行器驱动的近端催化发夹组装（P-CHA），一种可用于检测 TK1 mRNA 的 DNA 纳米传感器应运而生。P-CHA 发夹与链接 DNA 链杂交，并与磁性纳米粒子耦合以增加其局部浓度。双足 DNA 在 NPs 表面行走，利用邻近效应加速了反应动力学。利用 P-CHA 的信号放大作用以及 DNA 步行器在 80 分钟内的快速反应速度，所提出的传感器可以检测 TK1 mRNA，检测限低至 14 pM，可应用于临床诊断。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.