Tamir Partosh, Michael Davidovitz, Noa Firer, Gur Pines
{"title":"Stable clustered regularly interspaced short palindromic repeats‐based genome editing in Harmonia axyridis","authors":"Tamir Partosh, Michael Davidovitz, Noa Firer, Gur Pines","doi":"10.1111/jen.13332","DOIUrl":null,"url":null,"abstract":"<jats:italic>Harmonia axyridis</jats:italic> (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. In this study, we utilized the CRISPR‐Cas9 approach to achieve precise genome editing in <jats:italic>H</jats:italic>. <jats:italic>axyridis</jats:italic>. Specifically, we targeted two genes in <jats:italic>H</jats:italic>. <jats:italic>axyridis</jats:italic>, <jats:italic>laccase2</jats:italic> and <jats:italic>scarlet</jats:italic>, knockdown of which orthologues in other insects showed visible phenotypic changes. The knockout <jats:italic>laccase2</jats:italic> resulted not only in an early‐detectable phenotype but also in lethality. However, we successfully established a viable and genetically stable mutant colony by disrupting the <jats:italic>scarlet</jats:italic> gene, resulting in beetles with white eyes. Our findings contribute to the expanding knowledge of genetic manipulation in <jats:italic>H</jats:italic>. <jats:italic>axyridis</jats:italic> and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Entomology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/jen.13332","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. In this study, we utilized the CRISPR‐Cas9 approach to achieve precise genome editing in H. axyridis. Specifically, we targeted two genes in H. axyridis, laccase2 and scarlet, knockdown of which orthologues in other insects showed visible phenotypic changes. The knockout laccase2 resulted not only in an early‐detectable phenotype but also in lethality. However, we successfully established a viable and genetically stable mutant colony by disrupting the scarlet gene, resulting in beetles with white eyes. Our findings contribute to the expanding knowledge of genetic manipulation in H. axyridis and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.
期刊介绍:
The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems.
Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.