Serife Bekar, Tugba Sezgin Arslan, Yavuz Emre Arslan
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引用次数: 0
Abstract
In the present study, a method is proposed for preparing novel ductile-sticky materials that can be used as bone void fillers using hydrolyzed wool-keratin (WK) and silk fibroin (SF). This methodology uses citric acid as a cross-linking agent in preparing keratin paste (KP) owing to its non-toxicity and plasticizing properties. The Keratin paste-silk fibroin structure (KPSF) is obtained by adding SF, which possesses biocompatible and superior mechanical properties. Methanol treatment is employed on the KPSF mixture to convert the Silk I structure in the SF to Silk II, resulting in a water-insoluble and tightly packed proteinaceous structure. The physicochemical properties of both bioscaffolds are investigated and discussed in detail by comparison. Based on the findings, the presence of SF in the KPSF structure contributed to properties such as flexibility and porosity. In ovo CAM analysis reveals that both materials exhibit proangiogenic properties and are biocompatible. KP and KPSF bioscaffolds can be converted into ductile-sticky forms by adding water. It believes that these forms can easily apply to bone defect areas, particularly cavitary bone defects. Furthermore, KPSF bioscaffolds, with better mechanical properties, can be considered candidates for use in non-load-bearing bone tissue engineering applications.
本研究提出了一种利用水解羊毛角蛋白(WK)和丝纤维蛋白(SF)制备新型韧性粘性材料的方法,这种材料可用作骨空隙填充物。该方法使用柠檬酸作为交联剂来制备角蛋白浆糊(KP),因为柠檬酸具有无毒和可塑的特性。通过添加具有生物相容性和优异机械性能的 SF,可获得角蛋白浆-丝纤维蛋白结构(KPSF)。对 KPSF 混合物进行甲醇处理,将 SF 中的丝 I 结构转化为丝 II 结构,从而形成不溶于水且紧密结合的蛋白质结构。通过比较,对两种生物支架的理化特性进行了详细研究和讨论。根据研究结果,KPSF 结构中 SF 的存在有助于提高柔韧性和孔隙率等特性。体内 CAM 分析表明,这两种材料都具有促血管生成特性和生物相容性。KP 和 KPSF 生物支架可通过加水转化为韧性-粘性形式。该研究认为,这些形式可以很容易地应用于骨缺损区域,尤其是腔隙性骨缺损。此外,KPSF 生物支架具有更好的机械性能,可考虑用于非承重骨组织工程应用。
期刊介绍:
Macromolecular Materials and Engineering is the high-quality polymer science journal dedicated to the design, modification, characterization, processing and application of advanced polymeric materials, including membranes, sensors, sustainability, composites, fibers, foams, 3D printing, actuators as well as energy and electronic applications.
Macromolecular Materials and Engineering is among the top journals publishing original research in polymer science.
The journal presents strictly peer-reviewed Research Articles, Reviews, Perspectives and Comments.
ISSN: 1438-7492 (print). 1439-2054 (online).
Readership:Polymer scientists, chemists, physicists, materials scientists, engineers
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