Development and characterization of palmitoyl-modified arginine containing curcumin-loaded niosomal vesicles for improved antioxidant and anticancer activities

IF 2.2 4区 化学 Q3 CHEMISTRY, PHYSICAL
Khadija Rehman, Tasmina Kanwal, Ali Asgher Shuja, Salim Saifullah, Shabana Usman Simjee, Muhammad Raza Shah
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引用次数: 0

Abstract

The objective of this study was to enhance the anticancer potential of curcumin (CR)-loaded niosomal formulation against human myeloid leukemia cell lines and its antioxidant activity by the incorporation of amphiphilic palmitoyl-modified arginine (PL-ARG) serving as a positively charged amphiphile. PL-ARG was synthesized by using palmitoyl chloride (PL) and l-arginine (ARG) followed by characterization through ESI–MS, 1H-NMR, and FT-IR. The proportion of niosomal constituents including Tween 80/20 and cholesterol was optimized using experimental design to achieve small size vesicle with enhanced encapsulation of CR, and the optimized proportion was then used for the development of niosomal formulation functionalized with synthesized PL-ARG (PL-ARG-CR-NSMs). For the comparison, non-functionalized CR-loaded niosomes (CR-NSMs) were also prepared. The developed niosomal vesicles were characterized for encapsulation efficiency, size, PDI, zeta potential and surface morphology, and in vitro drug release. CR was encapsulated in niosomes with entrapment efficiency of 78.94 ± 5.16% (PL-ARG-CR-NSMs) and 65.82 ± 3.52% (CR-NSM) and provided a sustained drug release profile. Interestingly, when compared to CR-NSMs, the PL-ARG-CR-NSMs demonstrated enhanced growth inhibition of human myeloid leukemia cell lines supporting the antitumor efficacy of CR delivered from PL-ARG-CR-NSMs against human myeloid leukemia cell lines as well as the feasibility of arginine modified niosomes as a sustainable cancer treatment approach.

Graphical Abstract

Abstract Image

Abstract Image

开发和鉴定含有姜黄素的棕榈酰改性精氨酸载体,提高抗氧化和抗癌活性
本研究的目的是通过加入两亲性棕榈酰修饰精氨酸(PL-ARG)作为带正电荷的亲和剂,提高姜黄素(CR)载体纳米囊制剂对人类髓系白血病细胞株的抗癌潜力及其抗氧化活性。PL-ARG 是用棕榈酰氯(PL)和 l-精氨酸(ARG)合成的,然后通过 ESI-MS、1H-NMR 和 FT-IR 进行表征。通过实验设计优化了包括吐温 80/20 和胆固醇在内的尼索米尔成分的比例,以实现小尺寸囊泡并增强对 CR 的包封,然后利用优化后的比例开发了与合成的 PL-ARG 功能化的尼索米尔制剂(PL-ARG-CR-NSMs)。为了进行比较,还制备了非功能化的 CR 负载纳米囊(CR-NSMs)。对所制备的niosomal囊泡的包封效率、大小、PDI、zeta电位和表面形态以及体外药物释放进行了表征。CR被包裹在niosomes中,其包封效率为78.94 ± 5.16%(PL-ARG-CR-NSMs)和65.82 ± 3.52%(CR-NSM),并具有持续的药物释放特性。有趣的是,与 CR-NSMs 相比,PL-ARG-CR-NSMs 对人类髓性白血病细胞株的生长抑制作用更强,证明了 PL-ARG-CR-NSMs 释放的 CR 对人类髓性白血病细胞株的抗肿瘤疗效,以及精氨酸修饰的 niosomes 作为一种可持续癌症治疗方法的可行性。
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来源期刊
Colloid and Polymer Science
Colloid and Polymer Science 化学-高分子科学
CiteScore
4.60
自引率
4.20%
发文量
111
审稿时长
2.2 months
期刊介绍: Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.
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