Comprehensive proteomic characterization of urethral stricture disease in the Chinese population

IF 3.9 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Frontiers in Molecular Biosciences Pub Date : 2024-07-26 DOI:10.3389/fmolb.2024.1401970

Jiangtao Gao, Hui Liu, Lingling Li, Chunmei Guo, Zhiyong Wang, Mengya Cheng, Subei Tan, Lu Chen, Jijing Shi, Hui Wu, Chao Feng, Guoying Yu, Chen Ding

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Abstract

BackgroundMale urethral stricture disease (USD) is predominantly characterized by scar formation. There are few effective therapeutic drugs, and comprehensive molecular characterizations of USD formation remain undefined.MethodsThe proteomic profiling of twelve scar tissues and five matched normal adjacent tissues (NATs). Proteomic analysis methods were applied to explore the molecular characterizations of USD formation, including uncovering mechanistic pathways and providing novel biomarkers for scar formation.ResultsComparative proteomic analysis showed that the extracellular matrix (ECM) and complement cascade signaling were predominant in scar tissues. COL11A1 and CD248 significantly contributed to the accumulation of ECM components. Our study presented diverse molecular mechanisms of scar formation across different ages and suggested the potential effects of PXK in Age 1 (<45) patients. Furthermore, immune infiltration studies indicated the therapeutic potential of inhibiting the complement system (C4A, C4B) in Age 2 (≥45) patients, providing a potential clinical strategy for USD.ConclusionThis study illustrated the pathogenesis of USD formation and the diverse characteristics of USD patients with different ages, enhancing our understanding of the disease’s pathogenesis and providing a valuable resource for USD treatment.

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尿道狭窄疾病在中国人群中的综合蛋白质组特征分析

背景男性尿道狭窄症（USD）的主要特征是瘢痕形成。方法对 12 个瘢痕组织和 5 个匹配的正常邻近组织（NATs）进行蛋白质组学分析。结果比较蛋白质组分析表明，细胞外基质（ECM）和补体级联信号在瘢痕组织中占主导地位。COL11A1 和 CD248 对 ECM 成分的积累有重要作用。我们的研究提出了不同年龄段疤痕形成的多种分子机制，并提示了 PXK 对 1 岁 (<45) 患者的潜在作用。此外，免疫浸润研究表明，抑制补体系统（C4A、C4B）对年龄 2（≥45 岁）的患者具有治疗潜力，这为巩膜脓肿的临床治疗提供了一种潜在的策略。结论本研究阐述了巩膜脓肿形成的发病机制以及不同年龄巩膜脓肿患者的不同特征，加深了我们对该疾病发病机制的理解，为巩膜脓肿的治疗提供了宝贵的资源。

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来源期刊

Frontiers in Molecular Biosciences Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

7.20

自引率

4.00%

发文量

1361

审稿时长

14 weeks

期刊介绍： Much of contemporary investigation in the life sciences is devoted to the molecular-scale understanding of the relationships between genes and the environment — in particular, dynamic alterations in the levels, modifications, and interactions of cellular effectors, including proteins. Frontiers in Molecular Biosciences offers an international publication platform for basic as well as applied research; we encourage contributions spanning both established and emerging areas of biology. To this end, the journal draws from empirical disciplines such as structural biology, enzymology, biochemistry, and biophysics, capitalizing as well on the technological advancements that have enabled metabolomics and proteomics measurements in massively parallel throughput, and the development of robust and innovative computational biology strategies. We also recognize influences from medicine and technology, welcoming studies in molecular genetics, molecular diagnostics and therapeutics, and nanotechnology. Our ultimate objective is the comprehensive illustration of the molecular mechanisms regulating proteins, nucleic acids, carbohydrates, lipids, and small metabolites in organisms across all branches of life. In addition to interesting new findings, techniques, and applications, Frontiers in Molecular Biosciences will consider new testable hypotheses to inspire different perspectives and stimulate scientific dialogue. The integration of in silico, in vitro, and in vivo approaches will benefit endeavors across all domains of the life sciences.