Extraction, Isolation and Characterization of Bioactive Compounds from Euphorbia neriifolia (L.) Leaf and Evaluation of their Antioxidant Activity

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Current Analytical Chemistry Pub Date : 2024-07-23 DOI:10.2174/0115734110319220240718094750

Priya Chaudhary, Devendra Singh, Mukesh Meena, Pracheta Janmeda

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引用次数: 0

Abstract

Background: Amidst the identification of numerous secondary chemicals from Euphorbia neriifolia, there is a desperate need for the development of primary metabolite separation techniques. Objectives: In order to do that, bioactive chemicals from Euphorbia neriifolia (L.) leaf were extracted, isolated, and characterized. Subsequently, their antioxidant activity was evaluated. Methods: In this study, the determination of linoleic acid (LA) in petroleum ether extract (PEE) of Euphorbia neriifolia leaf (ENL) was carried out the first time by using thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) methods. Results: The chromatographic analysis of the PEE of ENL shows better spots and well-separated peak of LA with 0.49 retention factor (Rf) value and 22.54 ng LA content. The linearity of the calibration curve ranges from 5-25 ng/spot with a high correlation coefficient. The proposed method was characterized by better accuracy close to 99.5%, well robustness, and good precision range from 0.183% (intra-day) to 0.242% (inter-day). The percentage (%) RSD, which determined the stability of standard LA, did not exceed 2% after time period of 12, 24, 36, 48, and 72 h. The GC-MS analysis revealed the presence of different types of low or high-molecular-weight phytocompounds of varying quantities from the fractions of ENL. The FT-IR spectrum of ICs showed various peaks that confirmed the presence of C=C bending, C-H stretching, O-H stretching, CH2 stretching, and a carboxyl group. The 1H-NMR spectrum of the ICs from ENL confirmed the presence of octadecanoic in IC1, L-(+)-ascorbic acid dihexadecanoate in IC2, hexadecanoic acid in IC3, linoleic acid in IC4, and oleic acid in IC5, respectively. IC-4 showed greater antioxidant activity in comparison to other compounds with an IC50 value of 3.9 ± 0.01 μg mL-1. Conclusion: Thus, the present study identified five different phytocompounds that may be utilized as an effective option for the cure of different diseases.

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从 Euphorbia neriifolia（L. ）叶中提取、分离和表征生物活性化合物并评估其抗氧化活性

背景：在从 Euphorbia neriifolia 中鉴定出大量次级化学物质的同时，迫切需要开发初级代谢物分离技术。研究目的为此，我们提取、分离并鉴定了大戟科植物叶片中的生物活性化学物质。随后，对其抗氧化活性进行了评估。方法：本研究首次采用薄层色谱法（TLC）和高效薄层色谱法（HPTLC）测定了大戟科植物大戟叶（ENL）石油醚提取物（PEE）中的亚油酸（LA）。结果ENL的PEE色谱分析结果表明，LA的斑点较好，色谱峰分离度较高，保留因子（Rf）为0.49，LA含量为22.54 ng。校正曲线的线性范围为5-25 ng/spot，相关系数较高。该方法的准确度接近99.5%，稳健性好，精密度范围为0.183%（日内）至0.242%（日间）。在12、24、36、48和72 h内,标准LA的稳定性均不超过2%;GC-MS分析表明,ENL馏分中含有不同种类、不同含量的低分子量或高分子量植物化合物。集成电路的傅立叶变换红外光谱显示出不同的峰值，证实了 C=C 弯曲、C-H 拉伸、O-H 拉伸、CH2 拉伸和羧基的存在。ENL的ICs的1H-NMR光谱分别证实了IC1中含有十八酸，IC2中含有L-(+)-抗坏血酸二十六酸，IC3中含有十六酸，IC4中含有亚油酸，IC5中含有油酸。与其他化合物相比，IC-4 显示出更强的抗氧化活性，其 IC50 值为 3.9 ± 0.01 μg mL-1。结论因此，本研究发现了五种不同的植物化合物，可作为治疗不同疾病的有效选择。

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来源期刊

Current Analytical Chemistry 化学-分析化学

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Current Analytical Chemistry publishes full-length/mini reviews and original research articles on the most recent advances in analytical chemistry. All aspects of the field are represented, including analytical methodology, techniques, and instrumentation in both fundamental and applied research topics of interest to the broad readership of the journal. Current Analytical Chemistry strives to serve as an authoritative source of information in analytical chemistry and in related applications such as biochemical analysis, pharmaceutical research, quantitative biological imaging, novel sensors, and nanotechnology.