Complementarity between Brillouin signature and Scholte wave for controlled elasticity in fibrillated collagen medium for culture cell

Abstract

Modulating extracellular matrix (ECM) elasticity with fibrillar collagen offers great potential for regenerative medicine, drug discovery, and disease modeling by replicating in vivo mechanical signals. This enhances the understanding of cellular responses and fosters therapeutic innovation. However, precise ECM elasticity measurements are still lacking. This study couples time-resolved Brillouin spectroscopy and pulsed laser-induced Scholte wave generation. We measure how collagen fibrillation affects sound velocity and refractive index. These insights are advancing tissue engineering and cellular biomechanics.