A New Bacteriophage Pseudomonas Phage Ka1 from a Tributary Stream of Lake Baikal

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-26 DOI:10.1134/s0026261723604906

M. S. Fedorova, A. E. Gatina, V. N. Ilyina, L. L. Yadykova, V. V. Drukker, A. S. Gorshkova, A. R. Kayumov, E. Y. Trizna

引用次数: 0

Abstract

The bacteriophage Pseudomonas phage Ka1 was isolated from the water of a tributary stream of Lake Baikal, in the area of discharge of treated wastewater of the city of Slyudyanka. The bacteriophage genome contains 46 092 bp and has a 50% G+C pairs (AN OP455935.1). It exhibits 91% identity with the Pseudomonas phage PSA37 genome, belonging to Bruynoghevirus from the class Caudoviricetes suggesting that Ka1 is a new representative of Luz24-like phages (Bruynoghevirus). The bacteriophage lyzes 62% of P. aeruginosa clinical isolates and increases the efficiency of gentamicin, ciprofloxacin, imipenem and meropenem by 4–8-fold against these bacteria. Integrases, transposases and recombinases have not been identified in the genome of the bacteriophage Pseudomonas phage Ka1; which allows using it in the complex therapy of infections caused by P. aeruginosa.

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来自贝加尔湖支流的一种新的噬菌假单胞菌噬菌体 Ka1

摘要从斯柳江卡市废水处理排放区的贝加尔湖支流水中分离出了噬菌体假单胞菌噬菌体Ka1。噬菌体基因组包含 46 092 bp，G+C 对为 50%（AN OP455935.1）。它与假单胞菌噬菌体 PSA37 的基因组有 91% 的相同性，属于 Caudoviricetes 类布鲁诺格病毒，这表明 Ka1 是 Luz24 样噬菌体（布鲁诺格病毒）的新代表。这种噬菌体能溶解 62% 的铜绿假单胞菌临床分离株，并能将庆大霉素、环丙沙星、亚胺培南和美罗培南对这些细菌的杀菌效率提高 4-8 倍。在噬菌体假单胞菌噬菌体 Ka1 的基因组中尚未发现整合酶、转座酶和重组酶，因此可以将其用于铜绿假单胞菌感染的综合治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.