{"title":"Phylogenetic Analysis of phn Transporters of Achromobacter insolitus LCu2","authors":"Ye. V. Kryuchkova, G. L. Burygin","doi":"10.1134/s0026261723605122","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Phosphonates are alternative phosphorus sources for bacteria. The genome of <i>Achromobacter insolitus</i> strain LCu2 contains three predicted <i>phn</i> clusters of ABC-type phosphonate transporters into the cell. To understand the functional, evolutionary, and ecological role of the <i>phn</i> clusters, phylogenetic analysis of substrate-binding PhnD proteins from strain LCu2 with their homologs in other <i>Achromobacter</i> species and in closely related genera of the family <i>Alcaligenaceae</i> was carried out. The PhnD transporters formed three separate clusters, which indicated the differences in their structural composition. PhnD<sub>1</sub> and PhnD<sub>2</sub> were present in the genomes of all <i>Achromobacter</i> species and grouped separately from those of other members of the family <i>Alcaligenaceae</i>, which indicated vertical inheritance of the <i>phn</i>D<sub>1</sub> and <i>phn</i>D<sub>2</sub> genes and their involvement in the life-supporting processes. <i>Phn</i>D<sub>3</sub> was found in the genomes of seven <i>Achromobacter</i> species. The <i>phn</i>D<sub>3</sub> gene was probably acquired via horizontal transfer or duplication and is induced during adaptation to changing environmental conditions. Maintenance of three structurally different <i>phn</i> clusters of transporters is probably ecologically advantageous to <i>A. insolitus</i> LCu2, providing for phosphorus retrieval from synthetic and natural organophosphonates as well as other sources.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723605122","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Phosphonates are alternative phosphorus sources for bacteria. The genome of Achromobacter insolitus strain LCu2 contains three predicted phn clusters of ABC-type phosphonate transporters into the cell. To understand the functional, evolutionary, and ecological role of the phn clusters, phylogenetic analysis of substrate-binding PhnD proteins from strain LCu2 with their homologs in other Achromobacter species and in closely related genera of the family Alcaligenaceae was carried out. The PhnD transporters formed three separate clusters, which indicated the differences in their structural composition. PhnD1 and PhnD2 were present in the genomes of all Achromobacter species and grouped separately from those of other members of the family Alcaligenaceae, which indicated vertical inheritance of the phnD1 and phnD2 genes and their involvement in the life-supporting processes. PhnD3 was found in the genomes of seven Achromobacter species. The phnD3 gene was probably acquired via horizontal transfer or duplication and is induced during adaptation to changing environmental conditions. Maintenance of three structurally different phn clusters of transporters is probably ecologically advantageous to A. insolitus LCu2, providing for phosphorus retrieval from synthetic and natural organophosphonates as well as other sources.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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