Exploring discrepancies in muscle analysis with ImageJ: understanding the impact of tool selection on echo intensity and muscle area measurements.

IF 1.3 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Journal of Ultrasound Pub Date : 2024-12-01 Epub Date: 2024-07-26 DOI:10.1007/s40477-024-00934-4
Shabnam Lateef, Odessa Addison, Li-Qun Zhang, Vicki Gray, Marcel B Lanza
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引用次数: 0

Abstract

Purpose: The aim was to compare the use of different tools within the ImageJ program (polygon vs. segmented line) and their impact on the calculation of muscle area and echo intensity (EI) values in ultrasound imaging of the vastus lateralis muscle.

Methods: Thirteen volunteers participated in this study. Ultrasound images of the vastus lateralis muscle were acquired using 2D B-mode ultrasonography and analyzed using both the polygon and segmented line tools by the same evaluator. The intraclass correlation coefficient (ICC) and coefficient of variation (CV) assessed the tools' reliability. Bland-Altman plots were employed to verify the agreement between measurements, and linear regression analysis determined proportional bias. A paired t-test was conducted to analyze differences between the tools.

Results: The reliability between tools for muscle area calculation was weak (r = 0.000; CV = 138.03 ± 0.34%), while it was excellent for EI (r = 0.871; CV = 15.19 ± 2.96%). The Bland-Altman plots indicated a large bias for muscle area (d = 195.2%) with a proportional bias (p < 0.001). For EI, the bias was (d = 15.2) with proportional bias (p = 0.028). The paired t-test revealed significant differences between the tools for area (p < 0.001) but not for EI (p = 0.060).

Conclusion: The study found significant differences in measurements obtained with the polygon and segmented line tools in ImageJ, with the polygon tool showing higher values for muscle area and lower values for EI.

利用 ImageJ 探索肌肉分析中的差异:了解工具选择对回声强度和肌肉面积测量的影响。
目的:本研究旨在比较 ImageJ 程序中不同工具(多边形与分割线)的使用及其对阔筋膜外肌超声成像中肌肉面积和回声强度(EI)值计算的影响:13 名志愿者参与了这项研究。使用二维 B 型超声波采集了阔筋膜肌的超声波图像,并由同一评估人员使用多边形和分割线工具进行分析。类内相关系数(ICC)和变异系数(CV)评估了工具的可靠性。采用布兰-阿尔特曼图验证测量结果之间的一致性,并通过线性回归分析确定比例偏差。采用配对 t 检验分析工具之间的差异:肌肉面积计算工具之间的可靠性较弱(r = 0.000;CV = 138.03 ± 0.34%),而 EI 工具之间的可靠性极佳(r = 0.871;CV = 15.19 ± 2.96%)。布兰-阿尔特曼图显示,肌肉面积偏差较大(d = 195.2%),且存在比例偏差(p 结论):研究发现,使用 ImageJ 中的多边形工具和分割线工具进行的测量结果存在显著差异,多边形工具显示的肌肉面积值较高,而 EI 值较低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Ultrasound
Journal of Ultrasound RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
4.10
自引率
15.00%
发文量
133
期刊介绍: The Journal of Ultrasound is the official journal of the Italian Society for Ultrasound in Medicine and Biology (SIUMB). The journal publishes original contributions (research and review articles, case reports, technical reports and letters to the editor) on significant advances in clinical diagnostic, interventional and therapeutic applications, clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and in cross-sectional diagnostic imaging. The official language of Journal of Ultrasound is English.
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