Agar and agarose used for Staphylococcus aureus biofilm cultivation impact fluoroquinolone tolerance.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Angela D Power, Wendy W K Mok
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引用次数: 0

Abstract

Aims: Staphylococcus aureus is an opportunistic pathogen whose treatment is further complicated by its ability to form biofilms. In this study, we examine the impact of growing S. aureus biofilms on different polymerizing surfaces, specifically agar and agarose, on the pathogen's tolerance to fluoroquinolones.

Methods and results: Biofilms of two methicillin-resistant strains of S. aureus were grown on agar or agarose in the presence of the same added nutrients, and their antibiotic susceptibility to two fluoroquinolones, moxifloxacin (MXF) and delafloxacin (DLX), were measured. We also compared the metabolism and extracellular polymeric substances (EPS) production of biofilms that were grown on agar and agarose.

Conclusions: Biofilms that were grown on agarose were consistently more susceptible to antibiotics than those grown on agar. We found that in biofilms that were grown on agar, extracellular protein composition was higher, and adding EPS to agarose-grown biofilms increased their tolerance to DLX to levels that were comparable to agar-grown biofilms.

用于金黄色葡萄球菌生物膜培养的琼脂和琼脂糖会影响氟喹诺酮耐受性。
目的:金黄色葡萄球菌是一种机会性病原体,其形成生物膜的能力使治疗变得更加复杂。在本研究中,我们研究了金黄色葡萄球菌生物膜在不同聚合表面(特别是琼脂和琼脂糖)上生长对病原体耐氟喹诺酮类药物的影响:在琼脂或琼脂糖上生长两种耐甲氧西林金黄色葡萄球菌菌株的生物膜,同时添加相同的营养物质,并测定它们对两种氟喹诺酮类药物莫西沙星(MXF)和地氟沙星(DLX)的抗生素敏感性。我们还比较了在琼脂和琼脂糖上生长的生物膜的新陈代谢和细胞外聚合物质(EPS)产生情况:结论:在琼脂糖上生长的生物膜对抗生素的敏感性始终高于在琼脂糖上生长的生物膜。我们发现,在琼脂上生长的生物膜胞外蛋白质成分较高,在琼脂糖上生长的生物膜中添加 EPS 可提高生物膜对 DLX 的耐受性,使其达到与琼脂上生长的生物膜相当的水平。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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