Single-cell RNA sequencing of peripheral blood mononuclear cells from pregnant women with Systemic lupus erythematosus.

IF 4.3 4区 医学 Q2 IMMUNOLOGY
International Reviews of Immunology Pub Date : 2024-01-01 Epub Date: 2024-07-27 DOI:10.1080/08830185.2024.2376649
Congcong Liu, Zeyang Yu, Yijun Song, Xiaojie Zhang, Jiuliang Zhao, Qian Yu, Mengtao Li, Yuezhen Li, Juntao Liu
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引用次数: 0

Abstract

Systemic lupus erythematosus (SLE), an autoimmune condition, presents pregnancy-related risks, impacting maternal and fetal health. The immune cell composition and gene expression profiles in pregnant SLE patients, as well as the molecular mechanisms of active SLE patients during pregnancy, remain unclear. In our study, we enrolled 12 patients: three active SLE individuals (SLE-AT group, SLEDAI > 12, non-pregnant women), three inactive SLE individuals (SLE-NP group, SLEDAI ranging 0 to 6, non-pregnant women), three pregnant women with active SLE (SLE-C group, SLEDAI > 12), and three pregnant women with inactive SLE (SLE-NC group, SLEDAI range 0 to 6 score). Transcriptome analysis of peripheral blood mononuclear cells (PBMCs) was conducted using the 10x Genomics technique. We observed upregulation of genes like CCDC15 and TRBV4-2 in T cells and CMPK2, IFIT1, and OAS2 in monocytes in the SLE-C group. Notably, gene sets related to Cell Cycle and IFN Response showed significant differences between the SLE-C and SLE-NC groups in naïve CD8 T cells. Our comparison of immune cell type ratios and transcriptional patterns between active and inactive SLE during pregnancy sheds light on the single-cell level changes in SLE status during pregnancy, offering insights for future SLE prediction and treatment strategies.

对患有系统性红斑狼疮的孕妇的外周血单核细胞进行单细胞 RNA 测序。
系统性红斑狼疮(SLE)是一种自身免疫性疾病,具有与妊娠相关的风险,会影响母体和胎儿的健康。妊娠期系统性红斑狼疮患者的免疫细胞组成和基因表达谱以及活动性系统性红斑狼疮患者在妊娠期间的分子机制仍不清楚。在我们的研究中,我们招募了 12 名患者:3 名活动性系统性红斑狼疮患者(SLE-AT 组,SLEDAI > 12 分,非孕妇)、3 名非活动性系统性红斑狼疮患者(SLE-NP 组,SLEDAI 0-6 分,非孕妇)、3 名活动性系统性红斑狼疮孕妇(SLE-C 组,SLEDAI > 12 分)和 3 名非活动性系统性红斑狼疮孕妇(SLE-NC 组,SLEDAI 0-6 分)。我们使用 10x Genomics 技术对外周血单核细胞(PBMC)进行了转录组分析。我们观察到在 SLE-C 组中,T 细胞中的 CCDC15 和 TRBV4-2 等基因上调,单核细胞中的 CMPK2、IFIT1 和 OAS2 等基因上调。值得注意的是,在 SLE-C 组和 SLE-NC 组的幼稚 CD8 T 细胞中,与细胞周期和 IFN 反应相关的基因组显示出显著差异。我们对孕期活动性和非活动性系统性红斑狼疮的免疫细胞类型比例和转录模式进行的比较,揭示了孕期系统性红斑狼疮状态在单细胞水平上的变化,为未来系统性红斑狼疮的预测和治疗策略提供了启示。
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来源期刊
CiteScore
11.00
自引率
4.00%
发文量
24
期刊介绍: This review journal provides the most current information on basic and translational research in immunology and related fields. In addition to invited reviews, the journal accepts for publication articles and editorials on relevant topics proposed by contributors. Each issue of International Reviews of Immunology contains both solicited and unsolicited review articles, editorials, and ''In-this-Issue'' highlights. The journal also hosts reviews that position the authors'' original work relative to advances in a given field, bridging the gap between annual reviews and the original research articles. This review series is relevant to all immunologists, molecular biologists, microbiologists, translational scientists, industry researchers, and physicians who work in basic and clinical immunology, inflammatory and allergic diseases, vaccines, and additional topics relevant to medical research and drug development that connect immunology to disciplines such as oncology, cardiovascular disease, and metabolic disorders. Covered in International Reviews of Immunology: Basic and developmental immunology (innate and adaptive immunity; inflammation; and tumor and microbial immunology); Clinical research (mechanisms of disease in man pertaining to infectious diseases, autoimmunity, allergy, oncology / immunology); and Translational research (relevant to biomarkers, diagnostics, vaccines, and drug development).
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