Host-derived lactic acid bacteria alleviate short beak and dwarf syndrome by preventing bone loss, intestinal barrier disruption, and inflammation

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2024-07-19 DOI:10.1016/j.vetmic.2024.110187

Mandi Liu , Fengjun Xiang , Jialu Pan , Yongzhi Xue , Maoyuan Sun , Kuan Zhao , Wuchao Zhang , Baishi Lei , Peipei Gao , Limin Li , Wanzhe Yuan

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引用次数: 0

Abstract

Short-beak and dwarf syndrome (SBDS) is caused by novel goose parvovirus (NGPV) infection, which leads to farm economic losses. Our research aimed to investigate the potential of administering isolated lactic acid bacteria (LAB) in alleviating SBDS in ducks. Eight wild LAB strains were isolated from duck feces and their biosecurity was investigated in both duck embryo fibroblast (DEF) and live ducks. Moreover, the LAB strains exhibited no detrimental effects on bone metabolism levels and facilitated the tight junction proteins (TJPs) mRNA expression, and contributing to the mitigation of inflammation in healthy ducks. Subsequently, we conducted in vitrol and in vivo experiments to assess the impact of LAB on NGPV infection. The LAB strains significantly reduced the viral load of NGPV and downregulated the mRNA levels of pro-inflammatory factors in DEF. Additionally, LAB treatment alleviated SBDS in NGPV-infected ducks. Furthermore, LAB treatment alleviated intestinal damage, and reduced the inflammatory response, while also mitigating bone resorption in NGPV-infected ducks. In conclusion, the LAB strains isolated from duck feces have favorable biosecurity and alleviate SBDS in ducks, and the mechanism related to LAB improves intestinal barrier integrity, alleviates inflammation, and reduces bone resorption. Our study presents a novel concept for the prevention and treatment of NGPV, thereby establishing a theoretical foundation for the future development of probiotics in the prevention and treatment of NGPV.

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宿主源性乳酸菌可防止骨质流失、肠道屏障破坏和炎症，从而缓解短喙和侏儒综合症。

短喙矮小综合征（SBDS）是由新型鹅副粘病毒（NGPV）感染引起的，会导致农场经济损失。我们的研究旨在探讨给鸭子施用分离的乳酸菌（LAB）在缓解 SBDS 方面的潜力。我们从鸭粪中分离出八种野生 LAB 菌株，并在鸭胚胎成纤维细胞（DEF）和活鸭中对其生物安全性进行了研究。结果表明，这些LAB菌株对鸭的骨代谢水平无不利影响，并能促进鸭体内紧密连接蛋白（TJPs）mRNA的表达，有助于减轻健康鸭的炎症反应。随后，我们进行了体外和体内实验，以评估 LAB 对 NGPV 感染的影响。LAB菌株能明显降低NGPV的病毒载量，并下调DEF中促炎因子的mRNA水平。此外，LAB 处理可减轻 NGPV 感染鸭的 SBDS。此外，LAB 治疗还减轻了 NGPV 感染鸭的肠道损伤，降低了炎症反应，同时还减轻了骨吸收。总之，从鸭粪便中分离出的 LAB 菌株具有良好的生物安全性，可减轻鸭的 SBDS，LAB 的相关机制可改善肠道屏障的完整性、减轻炎症反应并减少骨吸收。我们的研究提出了预防和治疗 NGPV 的新概念，从而为益生菌在预防和治疗 NGPV 方面的未来发展奠定了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.