{"title":"Molecular characteristics and antibiotic resistance of Staphylococcus aureus and Staphylococcus haemolyticus isolated from bovine mastitis","authors":"Dobroslava Bujňáková, Lívia Karahutová","doi":"10.1016/j.rvsc.2024.105365","DOIUrl":null,"url":null,"abstract":"<div><p>Staphylococci are classified as one of the pathogens causing bovine mastitis that can pose not only an economic loss to the dairy farms, but a serious public-health threat based on their zoonotic potential.</p><p>We focused to monitor phenotypes of the isolated strains of <em>Staphylococcus aureus (S. aureus)</em> and <em>Staphylococcus haemolyticus (S. haemolyticus)</em> from milk of cows with clinical mastitis, including antibiotic resistance, biofilm forming ability and the presence of biofilm- and toxin- related genes.</p><p>From a total of 191 milk samples were identified as <em>S. aureus</em> – 12% (22 isolates) and <em>S. haemolyticus</em> – 6% (12 isolates). Automatic interpreted reading of the antibiogram evaluated potentially 12 isolates as methicillin-resistant <em>S. aureus</em> and methicillin-resistant coagulase-negative <em>Staphylococci</em>. Genotypically, the isolates were positive for <em>bla</em>Z and negative for <em>mec</em>A and <em>mec</em>C. Others important mechanisms were inducible macrolide-lincosamide-streptogramin B (iMLS<sub>B</sub>) resistance with presence of <em>msr</em>A, <em>erm</em>C, <em>vga</em>A.</p><p>The most detected biofilm-associated and toxins genes were <em>clf</em>A, <em>sdr</em>D, <em>sdr</em>E, <em>fnbpB</em>, <em>bbp</em>, <em>isd</em>A, <em>isd</em>B, <u><em>hla</em></u> and <em>see</em>. <em>S. aureus</em> isolates were subjected to spa typing. It turned out that despite the strains coming from different farms, they were either resistant or sensitive to antibiotic, were all of the same spa-type t 10035.</p><p>Our findings revealed the presence iMLS<sub>B</sub>, which, to our best knowledge, were described in Slovakian bovine staphylococci rarely. The majority of isolates were multidrug-resistant and carried multiple virulence genes, posing a potential public-health risk.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"177 ","pages":"Article 105365"},"PeriodicalIF":2.2000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in veterinary science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0034528824002315","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Staphylococci are classified as one of the pathogens causing bovine mastitis that can pose not only an economic loss to the dairy farms, but a serious public-health threat based on their zoonotic potential.
We focused to monitor phenotypes of the isolated strains of Staphylococcus aureus (S. aureus) and Staphylococcus haemolyticus (S. haemolyticus) from milk of cows with clinical mastitis, including antibiotic resistance, biofilm forming ability and the presence of biofilm- and toxin- related genes.
From a total of 191 milk samples were identified as S. aureus – 12% (22 isolates) and S. haemolyticus – 6% (12 isolates). Automatic interpreted reading of the antibiogram evaluated potentially 12 isolates as methicillin-resistant S. aureus and methicillin-resistant coagulase-negative Staphylococci. Genotypically, the isolates were positive for blaZ and negative for mecA and mecC. Others important mechanisms were inducible macrolide-lincosamide-streptogramin B (iMLSB) resistance with presence of msrA, ermC, vgaA.
The most detected biofilm-associated and toxins genes were clfA, sdrD, sdrE, fnbpB, bbp, isdA, isdB, hla and see. S. aureus isolates were subjected to spa typing. It turned out that despite the strains coming from different farms, they were either resistant or sensitive to antibiotic, were all of the same spa-type t 10035.
Our findings revealed the presence iMLSB, which, to our best knowledge, were described in Slovakian bovine staphylococci rarely. The majority of isolates were multidrug-resistant and carried multiple virulence genes, posing a potential public-health risk.
期刊介绍：
Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research.
The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally.
High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health.
Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.
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