Diversity of Babesia spp. in skunks from selected states in the United States of America.

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI:10.1051/parasite/2024043
Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley
{"title":"Diversity of Babesia spp. in skunks from selected states in the United States of America.","authors":"Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley","doi":"10.1051/parasite/2024043","DOIUrl":null,"url":null,"abstract":"<p><p>Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale putorius, n = 28), western spotted skunk (Spilogale gracilis, n = 15), and hog-nosed skunk (Conepatus leuconotus, n = 11). A PCR assay targeting the 18S rRNA region and cox1 region were used to determine if skunks were infected with piroplasms and for phylogenetic analyses. A total of 48.4% (61/126) of skunks tested positive for a Babesia species. Both the 18S and cox1 analysis supported a skunk-specific Babesia microti-like sp. of carnivores as well as a species in the B. microti complex that is phylogenetically unique from both B. microti of humans and the B. microti-like sp. of carnivores. In the 18S analysis, there was a third species of Babesia in hog-nosed skunks in the western piroplasm group. This study shows that at least three species of piroplasms occur in skunk species in the United States and further highlights the importance of phylogenetic analyses and the use of multiple gene targets when studying piroplasms.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271706/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasite","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1051/parasite/2024043","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/24 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale putorius, n = 28), western spotted skunk (Spilogale gracilis, n = 15), and hog-nosed skunk (Conepatus leuconotus, n = 11). A PCR assay targeting the 18S rRNA region and cox1 region were used to determine if skunks were infected with piroplasms and for phylogenetic analyses. A total of 48.4% (61/126) of skunks tested positive for a Babesia species. Both the 18S and cox1 analysis supported a skunk-specific Babesia microti-like sp. of carnivores as well as a species in the B. microti complex that is phylogenetically unique from both B. microti of humans and the B. microti-like sp. of carnivores. In the 18S analysis, there was a third species of Babesia in hog-nosed skunks in the western piroplasm group. This study shows that at least three species of piroplasms occur in skunk species in the United States and further highlights the importance of phylogenetic analyses and the use of multiple gene targets when studying piroplasms.

美国部分州臭鼬中巴贝斯菌属的多样性。
巴贝西亚原虫是一种红细胞内原生动物寄生虫,可感染多种宿主。本研究的目的是评估美国各州臭鼬体内的巴贝西亚原虫种类,并确定是否存在地域差异。我们从宾夕法尼亚州、肯塔基州、北卡罗来纳州、南卡罗来纳州、佐治亚州、密苏里州、路易斯安那州、德克萨斯州、堪萨斯州和加利福尼亚州采集了脾脏、全血或滤纸上的血液,并对其进行了巴贝西亚原虫检测。我们检测了四种臭鼬,包括条纹臭鼬(Mephitis mephitis,n = 72)、东部斑点臭鼬(Spilogale putorius,n = 28)、西部斑点臭鼬(Spilogale gracilis,n = 15)和猪鼻臭鼬(Conepatus leuconotus,n = 11)。利用针对 18S rRNA 区域和 cox1 区域的 PCR 检测来确定臭鼬是否感染了皮罗浆虫,并进行系统发育分析。共有 48.4% 的臭鼬(61/126)对巴贝西亚原虫检测呈阳性。18S 和 cox1 分析都支持食肉动物中的臭鼬特异性微小巴贝西亚原虫,以及微小巴贝西亚原虫复合体中的一个物种,该物种在系统发育上既不同于人类的微小巴贝西亚原虫,也不同于食肉动物的微小巴贝西亚原虫。在 18S 分析中,猪鼻鼬中还有第三种巴贝西亚原虫,属于西部 piroplasm 组。这项研究表明,在美国的臭鼬物种中至少存在三种嗜血杆菌,并进一步强调了在研究嗜血杆菌时进行系统发育分析和使用多基因靶标的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信