Diversity of Babesia spp. in skunks from selected states in the United States of America.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI:10.1051/parasite/2024043
Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley
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引用次数: 0

Abstract

Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale putorius, n = 28), western spotted skunk (Spilogale gracilis, n = 15), and hog-nosed skunk (Conepatus leuconotus, n = 11). A PCR assay targeting the 18S rRNA region and cox1 region were used to determine if skunks were infected with piroplasms and for phylogenetic analyses. A total of 48.4% (61/126) of skunks tested positive for a Babesia species. Both the 18S and cox1 analysis supported a skunk-specific Babesia microti-like sp. of carnivores as well as a species in the B. microti complex that is phylogenetically unique from both B. microti of humans and the B. microti-like sp. of carnivores. In the 18S analysis, there was a third species of Babesia in hog-nosed skunks in the western piroplasm group. This study shows that at least three species of piroplasms occur in skunk species in the United States and further highlights the importance of phylogenetic analyses and the use of multiple gene targets when studying piroplasms.

美国部分州臭鼬中巴贝斯菌属的多样性。
巴贝西亚原虫是一种红细胞内原生动物寄生虫,可感染多种宿主。本研究的目的是评估美国各州臭鼬体内的巴贝西亚原虫种类,并确定是否存在地域差异。我们从宾夕法尼亚州、肯塔基州、北卡罗来纳州、南卡罗来纳州、佐治亚州、密苏里州、路易斯安那州、德克萨斯州、堪萨斯州和加利福尼亚州采集了脾脏、全血或滤纸上的血液,并对其进行了巴贝西亚原虫检测。我们检测了四种臭鼬,包括条纹臭鼬(Mephitis mephitis,n = 72)、东部斑点臭鼬(Spilogale putorius,n = 28)、西部斑点臭鼬(Spilogale gracilis,n = 15)和猪鼻臭鼬(Conepatus leuconotus,n = 11)。利用针对 18S rRNA 区域和 cox1 区域的 PCR 检测来确定臭鼬是否感染了皮罗浆虫,并进行系统发育分析。共有 48.4% 的臭鼬(61/126)对巴贝西亚原虫检测呈阳性。18S 和 cox1 分析都支持食肉动物中的臭鼬特异性微小巴贝西亚原虫,以及微小巴贝西亚原虫复合体中的一个物种,该物种在系统发育上既不同于人类的微小巴贝西亚原虫,也不同于食肉动物的微小巴贝西亚原虫。在 18S 分析中,猪鼻鼬中还有第三种巴贝西亚原虫,属于西部 piroplasm 组。这项研究表明,在美国的臭鼬物种中至少存在三种嗜血杆菌,并进一步强调了在研究嗜血杆菌时进行系统发育分析和使用多基因靶标的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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