Epigenomic differences between osteoarthritis grades in primary cartilage

IF 7.2 2区医学 Q1 ORTHOPEDICS

Osteoarthritis and Cartilage Pub Date : 2024-07-23 DOI:10.1016/j.joca.2024.07.008

Peter Kreitmaier , Diane Swift , J. Mark Wilkinson , Eleftheria Zeggini

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引用次数: 0

Abstract

Objective

Osteoarthritis is a common and complex joint disorder that shows higher prevalence and greater disease severity in women. Here, we investigate genome-wide methylation profiles of primary chondrocytes from osteoarthritis patients.

Design

We compare genome-wide methylation profiles of macroscopically intact (low-grade) and degraded (high-grade) osteoarthritis cartilage samples matched from osteoarthritis patients undergoing knee replacement surgery. We perform an epigenome-wide association study for cartilage degeneration across 170 patients and separately in 96 women and 74 men.

Results

We reveal widespread epigenetic differences with enrichments of nervous system and apoptosis-related processes. We further identify substantial similarities between sexes, but also sex-specific markers and pathways.

Conclusions

Together, we provide the largest genome-wide methylation profiles of primary cartilage to date with enhanced and sex-specific insights into epigenetic processes underlying osteoarthritis progression.

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原发性软骨中不同等级骨关节炎的表观基因组差异

目的：骨关节炎是一种常见且复杂的关节疾病，女性发病率更高，病情也更严重。在此，我们研究了骨关节炎患者原发性软骨细胞的全基因组甲基化图谱：我们比较了与接受膝关节置换手术的骨关节炎患者相匹配的宏观完整（低级）和退化（高级）骨关节炎软骨样本的全基因组甲基化图谱。我们对170名患者中的96名女性和74名男性分别进行了软骨退化表观基因组关联研究（EWAS）：结果：我们发现了广泛的表观遗传学差异，其中神经系统和细胞凋亡相关过程的差异较大。结果：我们发现了广泛的表观遗传学差异，其中神经系统和细胞凋亡相关过程富集，我们还发现了两性之间的大量相似性，以及性别特异性标记和通路：总之，我们提供了迄今为止最大规模的原发性软骨全基因组甲基化图谱，增强了对骨关节炎进展的表观遗传过程的洞察力，并具有性别特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Osteoarthritis and Cartilage 医学-风湿病学

CiteScore

11.70

自引率

7.10%

发文量

802

审稿时长

52 days

期刊介绍： Osteoarthritis and Cartilage is the official journal of the Osteoarthritis Research Society International. It is an international, multidisciplinary journal that disseminates information for the many kinds of specialists and practitioners concerned with osteoarthritis.