Genetically engineered synthetic cells activate cargo release upon temperature shift

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2024-07-25 DOI:10.1038/s41589-024-01701-6

引用次数: 0

Abstract

We combine RNA thermometer genetic switches, cell-free protein expression and synthetic cell design to create cell-sized systems that can initiate the synthesis of soluble proteins at defined temperatures. We show that when these switches are used to control the expression of a pore-forming membrane protein, temperature-controlled cargo release is achieved, with potential future applications in biomedicine.

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基因工程合成细胞在温度变化时激活货物释放。

我们将 RNA 温度计基因开关、无细胞蛋白质表达和合成细胞设计结合起来，创造出细胞大小的系统，可在规定温度下启动可溶性蛋白质的合成。我们的研究表明，当这些开关用于控制孔形成膜蛋白的表达时，就能实现温控货物释放，这在未来的生物医学中具有潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.