Characterization of Leclercia adecarboxylata isolated from field collected Anopheles subpictus, Berhampur, Odisha.

IF 0.8 4区医学 Q4 INFECTIOUS DISEASES

Journal of Vector Borne Diseases Pub Date : 2024-07-12 DOI:10.4103/JVBD.JVBD_22_24

Ankita Agrawal, Tapan Kumar Barik, Amiya Kumar Patel

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引用次数: 0

Abstract

Background objectives: Malaria, a vector-borne diseases caused by Plasmodium species and transmitted by Anopheles species. Among them, Anopheles subpictus has emerged as a potent malarial vector in coastal areas of India. Numerous studies highlighted that bacterial communities within mosquito influence vector competence. The present study was designed to isolate and characterize bacterial microbiota from A. subpictus larvae.

Methods: Isolation and purification of the predominant bacterial strain (AL1). Morphological, biochemical, antibiotic susceptibility and molecular characterization of the isolated bacteria.

Results: Bacterial isolate (AL1) was found to be rod, gram negative, catalase positive and oxidase negative. AL1 was identified as Leclercia adecarboxylata (Accession number: OR649235) through 16S rRNA ribotyping. Further, leaf extract of Nyctanthes arbortristis showed inhibitory effect against AL1.

Interpretation conclusion: This is the first report on isolation of symbiotic bacteria (L. adecarboxylata) from A. subpictus and its control by leaf extract of Nyctanthes arbortristis. Isolated gram-negative bacterial strain might inhibit the development of mosquito vector and might use for various biological control strategies to combat malaria transmission.

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从奥迪沙伯汉普尔野外采集的亚爪疟蚊中分离出的 Leclercia adecarboxylata 的特征。

背景目标：疟疾是由疟原虫引起的病媒传染病，由按蚊传播。其中，亚按蚊（Anopheles subpictus）已成为印度沿海地区强有力的疟疾病媒。大量研究强调，蚊子体内的细菌群落会影响病媒的能力。本研究旨在分离和描述亚按蚊幼虫体内的细菌微生物群：方法：分离和纯化主要细菌菌株（AL1）。对分离的细菌进行形态学、生物化学、抗生素敏感性和分子鉴定：结果：分离出的细菌（AL1）为杆状、革兰氏阴性、过氧化氢酶阳性和氧化酶阴性。通过 16S rRNA 核型鉴定，AL1 被确定为 Leclercia adecarboxylata（登录号：OR649235）。此外，Nyctanthes arbortristis 的叶提取物对 AL1 有抑制作用：这是首次报道从亚纲蝇蛆中分离出共生细菌（L. adecarboxylata），并用熊果叶提取物对其进行控制。分离到的革兰氏阴性细菌菌株可能会抑制蚊媒的发展，并可用于各种生物防治策略以对抗疟疾传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vector Borne Diseases INFECTIOUS DISEASES-PARASITOLOGY

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： National Institute of Malaria Research on behalf of Indian Council of Medical Research (ICMR) publishes the Journal of Vector Borne Diseases. This Journal was earlier published as the Indian Journal of Malariology, a peer reviewed and open access biomedical journal in the field of vector borne diseases. The Journal publishes review articles, original research articles, short research communications, case reports of prime importance, letters to the editor in the field of vector borne diseases and their control.