{"title":"An overview of analytical methods for detecting endogenous hydrogen sulfide (H2S) in plants","authors":"Huihui Fang, Yunxiang Zang","doi":"10.1016/j.jplph.2024.154315","DOIUrl":null,"url":null,"abstract":"<div><p>The significance of hydrogen sulfide (H<sub>2</sub>S) as a crucial gasotransmitter has been shown extensively in plants, and endogenous H<sub>2</sub>S is often modulated to activate H<sub>2</sub>S signaling when plants respond to numerous developmental and environmental cues. Consequently, elucidating the H<sub>2</sub>S physiological concentrations and the H<sub>2</sub>S generation intensity of plants is key to understanding the activation mechanism of H<sub>2</sub>S signaling, which has attracted increasing attention. Currently, a variety of reaction-based methods have been reported for monitoring H<sub>2</sub>S concentration <em>in vivo</em> and <em>in vitro</em>. In this review, we summarize and describe in detail several methods for quantifying and bioimaging endogenous H<sub>2</sub>S in plants systems, mainly the spectrophotometer-dependent methylene blue (MB) method and fluorescence probes, including the reaction mechanisms, design strategies, response principles, and application details. Moreover, we also summarize the advantages and disadvantages of these methods as well as the research scenarios in which they are applicable. We expect that this review will provide some guidelines on the selection of methods for H<sub>2</sub>S sensing and the comprehensive investigations into H<sub>2</sub>S signaling in plants.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"302 ","pages":"Article 154315"},"PeriodicalIF":4.0000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724001469","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The significance of hydrogen sulfide (H2S) as a crucial gasotransmitter has been shown extensively in plants, and endogenous H2S is often modulated to activate H2S signaling when plants respond to numerous developmental and environmental cues. Consequently, elucidating the H2S physiological concentrations and the H2S generation intensity of plants is key to understanding the activation mechanism of H2S signaling, which has attracted increasing attention. Currently, a variety of reaction-based methods have been reported for monitoring H2S concentration in vivo and in vitro. In this review, we summarize and describe in detail several methods for quantifying and bioimaging endogenous H2S in plants systems, mainly the spectrophotometer-dependent methylene blue (MB) method and fluorescence probes, including the reaction mechanisms, design strategies, response principles, and application details. Moreover, we also summarize the advantages and disadvantages of these methods as well as the research scenarios in which they are applicable. We expect that this review will provide some guidelines on the selection of methods for H2S sensing and the comprehensive investigations into H2S signaling in plants.
期刊介绍：
The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication.
The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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