Narjes Ahmadian, Maaike M Konig, Sigrid Otto, Kiki Tesselaar, Pieter van Eijsden, Mark Gosselink, Ayhan Gursan, Dennis W Klomp, Jeanine J Prompers, Evita C Wiegers
{"title":"Human Brain Deuterium Metabolic Imaging at 7 T: Impact of Different [6,6'-<sup>2</sup>H<sub>2</sub>]Glucose Doses.","authors":"Narjes Ahmadian, Maaike M Konig, Sigrid Otto, Kiki Tesselaar, Pieter van Eijsden, Mark Gosselink, Ayhan Gursan, Dennis W Klomp, Jeanine J Prompers, Evita C Wiegers","doi":"10.1002/jmri.29532","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Deuterium metabolic imaging (DMI) is an innovative, noninvasive metabolic MR imaging method conducted after administration of <sup>2</sup>H-labeled substrates. DMI after [6,6'-<sup>2</sup>H<sub>2</sub>]glucose consumption has been used to investigate brain metabolic processes, but the impact of different [6,6'-<sup>2</sup>H<sub>2</sub>]glucose doses on DMI brain data is not well known.</p><p><strong>Purpose: </strong>To investigate three different [6,6'-<sup>2</sup>H<sub>2</sub>]glucose doses for DMI in the human brain at 7 T.</p><p><strong>Study type: </strong>Prospective.</p><p><strong>Population: </strong>Six healthy participants (age: 28 ± 8 years, male/female: 3/3).</p><p><strong>Field strength/sequence: </strong>7 T, 3D <sup>2</sup>H free-induction-decay (FID)-magnetic resonance spectroscopic imaging (MRSI) sequence.</p><p><strong>Assessment: </strong>Three subjects received two different doses (0.25 g/kg, 0.50 g/kg or 0.75 g/kg body weight) of [6,6'-<sup>2</sup>H<sub>2</sub>]glucose on two occasions and underwent consecutive <sup>2</sup>H-MRSI scans for 120 minutes. Blood was sampled every 10 minutes during the scan, to determine plasma glucose levels and plasma <sup>2</sup>H-Glucose atom percent excess (APE) (part-1). Three subjects underwent the same protocol once after receiving 0.50 g/kg [6,6'-<sup>2</sup>H<sub>2</sub>]glucose (part-2).</p><p><strong>Statistical test: </strong>Mean plasma <sup>2</sup>H-Glucose APE and glucose plasma concentrations were compared using one-way ANOVA. Brain <sup>2</sup>H-Glc and brain <sup>2</sup>H-Glx (part-1) were analyzed with a two-level Linear Mixed Model. In part-2, a General Linear Model was used to compare brain metabolite signals. Statistical significance was set at P < 0.05.</p><p><strong>Results: </strong>Between 60 and 100 minutes after ingesting [6,6'-<sup>2</sup>H<sub>2</sub>]glucose, plasma <sup>2</sup>H-Glc APE did not differ between 0.50 g/kg and 0.75 g/kg doses (P = 0.961), but was significantly lower for 0.25 g/kg. Time and doses significantly affected brain <sup>2</sup>H-Glucose levels (estimate ± standard error [SE]: 0.89 ± 0.01, 1.09 ± 0.01, and 1.27 ± 0.01, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively) and brain <sup>2</sup>H-Glutamate/Glutamine levels (estimate ± SE: 1.91 ± 0.03, 2.27 ± 0.03, and 2.46 ± 0.03, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively). Plasma <sup>2</sup>H-Glc APE, brain <sup>2</sup>H-Glc, and brain <sup>2</sup>H-Glx levels were comparable among subjects receiving 0.50 g/kg [6,6'-<sup>2</sup>H<sub>2</sub>]glucose.</p><p><strong>Data conclusion: </strong>Brain <sup>2</sup>H-Glucose and brain <sup>2</sup>H-Glutamate/Glutamine showed to be [6,6'-<sup>2</sup>H<sub>2</sub>]glucose dose dependent. A dose of 0.50 g/kg demonstrated comparable, and well-detectable, <sup>2</sup>H-Glucose and <sup>2</sup>H-Glutamate/Glutamine signals in the brain.</p><p><strong>Evidence level: </strong>1 TECHNICAL EFFICACY: Stage 2.</p>","PeriodicalId":16140,"journal":{"name":"Journal of Magnetic Resonance Imaging","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Magnetic Resonance Imaging","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/jmri.29532","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Deuterium metabolic imaging (DMI) is an innovative, noninvasive metabolic MR imaging method conducted after administration of 2H-labeled substrates. DMI after [6,6'-2H2]glucose consumption has been used to investigate brain metabolic processes, but the impact of different [6,6'-2H2]glucose doses on DMI brain data is not well known.
Purpose: To investigate three different [6,6'-2H2]glucose doses for DMI in the human brain at 7 T.
Study type: Prospective.
Population: Six healthy participants (age: 28 ± 8 years, male/female: 3/3).
Field strength/sequence: 7 T, 3D 2H free-induction-decay (FID)-magnetic resonance spectroscopic imaging (MRSI) sequence.
Assessment: Three subjects received two different doses (0.25 g/kg, 0.50 g/kg or 0.75 g/kg body weight) of [6,6'-2H2]glucose on two occasions and underwent consecutive 2H-MRSI scans for 120 minutes. Blood was sampled every 10 minutes during the scan, to determine plasma glucose levels and plasma 2H-Glucose atom percent excess (APE) (part-1). Three subjects underwent the same protocol once after receiving 0.50 g/kg [6,6'-2H2]glucose (part-2).
Statistical test: Mean plasma 2H-Glucose APE and glucose plasma concentrations were compared using one-way ANOVA. Brain 2H-Glc and brain 2H-Glx (part-1) were analyzed with a two-level Linear Mixed Model. In part-2, a General Linear Model was used to compare brain metabolite signals. Statistical significance was set at P < 0.05.
Results: Between 60 and 100 minutes after ingesting [6,6'-2H2]glucose, plasma 2H-Glc APE did not differ between 0.50 g/kg and 0.75 g/kg doses (P = 0.961), but was significantly lower for 0.25 g/kg. Time and doses significantly affected brain 2H-Glucose levels (estimate ± standard error [SE]: 0.89 ± 0.01, 1.09 ± 0.01, and 1.27 ± 0.01, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively) and brain 2H-Glutamate/Glutamine levels (estimate ± SE: 1.91 ± 0.03, 2.27 ± 0.03, and 2.46 ± 0.03, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively). Plasma 2H-Glc APE, brain 2H-Glc, and brain 2H-Glx levels were comparable among subjects receiving 0.50 g/kg [6,6'-2H2]glucose.
Data conclusion: Brain 2H-Glucose and brain 2H-Glutamate/Glutamine showed to be [6,6'-2H2]glucose dose dependent. A dose of 0.50 g/kg demonstrated comparable, and well-detectable, 2H-Glucose and 2H-Glutamate/Glutamine signals in the brain.
期刊介绍：
The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.
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