Ana Clara Kuerten Gil, Eugenio A D Merino, Diogo Pontes Costa, César Nunes Giracca, Ricardo Mazzon, Gabriel Leonardo Magrin, Josiane de Almeida, Cesar Augusto Magalhães Benfatti
{"title":"A Novel Device for the Evaluation of In Vitro Bacterial Colonization in Membranes for Guided Tissue and Bone Regeneration.","authors":"Ana Clara Kuerten Gil, Eugenio A D Merino, Diogo Pontes Costa, César Nunes Giracca, Ricardo Mazzon, Gabriel Leonardo Magrin, Josiane de Almeida, Cesar Augusto Magalhães Benfatti","doi":"10.3390/dj12070202","DOIUrl":null,"url":null,"abstract":"<p><p><b>Purpose:</b> To evaluate, in vitro, the efficiency of a novel apparatus to test the adherence and penetration of bacteria on different membranes for guided regeneration. <b>Methodology:</b> To create the 3D device, Computer Aided Design/Computer Aided Manufacturing (CAD/CAM) systems were used. Three types of biomaterials were tested (<i>n</i> = 6): (DT) a collagen membrane; (DS) a polymer membrane; and (LP) a dense polytetrafluoroethylene barrier. The biomaterials were adapted to the apparatuses and challenged with two different monospecies bacterial culture of <i>A. actinomycetemcomitans b</i> and <i>S. mutans</i>. After 2 h, bacterial adherence and penetration were quantified by counting the number of colony-forming units (CFUs). Two specimens from each group were used for image analysis using Confocal Laser Scanning Microscopy. Statistical analysis was performed. <b>Findings:</b> The DS group had a higher adherence of <i>S. mutans</i> compared to <i>A. actinomycetemcomitans b</i> (<i>p</i> = 0.05). There was less adherence of <i>A. actinomycetemcomitans b</i> in the DS group, compared to the LP (<i>p</i> = 0.011) and DT (<i>p</i> < 0.001) groups. Only the membranes allowed penetration, which was blocked by barriers. The DT group allowed a greater penetration of <i>S. mutans</i> to occur compared to <i>A. actinomycetemcomitans b</i> (<i>p</i> = 0.009), which showed a higher penetration into the DS membranes compared to <i>S. mutans</i> (<i>p</i> = 0.016). The penetration of <i>A. actinomycetemcomitans b</i> through DS was higher compared to its penetration through DT and LP (<i>p</i> < 0.01 for both). DT and DS allowed a greater penetration of <i>S. mutans</i> to occur compared to LP, which prevented both bacterial species from penetrating. <b>Conclusion:</b> The apparatus allowed for the settlement and complete sealing of the biomaterials, enabling standardization.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11275268/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dentistry Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/dj12070202","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: To evaluate, in vitro, the efficiency of a novel apparatus to test the adherence and penetration of bacteria on different membranes for guided regeneration. Methodology: To create the 3D device, Computer Aided Design/Computer Aided Manufacturing (CAD/CAM) systems were used. Three types of biomaterials were tested (n = 6): (DT) a collagen membrane; (DS) a polymer membrane; and (LP) a dense polytetrafluoroethylene barrier. The biomaterials were adapted to the apparatuses and challenged with two different monospecies bacterial culture of A. actinomycetemcomitans b and S. mutans. After 2 h, bacterial adherence and penetration were quantified by counting the number of colony-forming units (CFUs). Two specimens from each group were used for image analysis using Confocal Laser Scanning Microscopy. Statistical analysis was performed. Findings: The DS group had a higher adherence of S. mutans compared to A. actinomycetemcomitans b (p = 0.05). There was less adherence of A. actinomycetemcomitans b in the DS group, compared to the LP (p = 0.011) and DT (p < 0.001) groups. Only the membranes allowed penetration, which was blocked by barriers. The DT group allowed a greater penetration of S. mutans to occur compared to A. actinomycetemcomitans b (p = 0.009), which showed a higher penetration into the DS membranes compared to S. mutans (p = 0.016). The penetration of A. actinomycetemcomitans b through DS was higher compared to its penetration through DT and LP (p < 0.01 for both). DT and DS allowed a greater penetration of S. mutans to occur compared to LP, which prevented both bacterial species from penetrating. Conclusion: The apparatus allowed for the settlement and complete sealing of the biomaterials, enabling standardization.