Abdulmajeed Alqasoumi, Mansour Alsharidah, Amer Mahmood, Mona Elsafadi, Osamah Al Rugaie, Khalid M Mohany, Khalid A Al-Regaiey, Khaleel I Alyahya, Alaa A Alanteet, Norah K Algarzae, Hanan K AlGhibiwi, Adel AlHomaidi, Mohammad Abumaree
{"title":"Mesenchymal Stem Cell-Conditioned Media Modulate HUVEC Response to H<sub>2</sub>O<sub>2</sub>: Impact on Gene Expression and Potential for Atherosclerosis Intervention.","authors":"Abdulmajeed Alqasoumi, Mansour Alsharidah, Amer Mahmood, Mona Elsafadi, Osamah Al Rugaie, Khalid M Mohany, Khalid A Al-Regaiey, Khaleel I Alyahya, Alaa A Alanteet, Norah K Algarzae, Hanan K AlGhibiwi, Adel AlHomaidi, Mohammad Abumaree","doi":"10.1155/2024/7726493","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> We studied the potential of human bone marrow-derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial cell properties (viability, proliferation, and migrations) from the deleterious effects produced by the inflammatory environment of H<sub>2</sub>O<sub>2</sub>. Additionally, we investigated their impact on the endothelial cells' gene expression of some inflammatory-related genes, namely, TGF-<i>β</i>1, FOS, ATF3, RAF-1, and SMAD3. <b>Methods:</b> Human umbilical vein endothelial cells (HUVECs) were cultured individually under three conditions: alone, with varying concentrations of H<sub>2</sub>O<sub>2</sub>, or with varying concentrations of H<sub>2</sub>O<sub>2</sub> and hBMSC CM. HUVEC adhesion, proliferation, and migration were evaluated using the xCELLigence system. The HUVECs' gene expressions were evaluated by real-time polymerase chain reaction (RT-PCR). <b>Results:</b> Generally, we observed enhanced HUVEC viability, proliferation, and migration when cultured in media supplemented with H<sub>2</sub>O<sub>2</sub> and hBMSC CM. Furthermore, the CM modulated the expressions of the studied inflammatory-related genes in HUVECs, promoting a more robust cellular response. <b>Conclusion:</b> This study has illuminated the protective role of hBMSC CM in mitigating the damaging effects of H<sub>2</sub>O<sub>2</sub> on endothelial cell function. Our data demonstrate that hBMSC CM enhances the viability, proliferation, and migration of HUVECs even under oxidative stress conditions. Additionally, the conditioned medium was found to modulate the gene expression of pivotal markers related to inflammation, suggesting a favorable influence on cellular response mechanisms.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"7726493"},"PeriodicalIF":2.6000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268959/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioMed Research International","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2024/7726493","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: We studied the potential of human bone marrow-derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial cell properties (viability, proliferation, and migrations) from the deleterious effects produced by the inflammatory environment of H2O2. Additionally, we investigated their impact on the endothelial cells' gene expression of some inflammatory-related genes, namely, TGF-β1, FOS, ATF3, RAF-1, and SMAD3. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured individually under three conditions: alone, with varying concentrations of H2O2, or with varying concentrations of H2O2 and hBMSC CM. HUVEC adhesion, proliferation, and migration were evaluated using the xCELLigence system. The HUVECs' gene expressions were evaluated by real-time polymerase chain reaction (RT-PCR). Results: Generally, we observed enhanced HUVEC viability, proliferation, and migration when cultured in media supplemented with H2O2 and hBMSC CM. Furthermore, the CM modulated the expressions of the studied inflammatory-related genes in HUVECs, promoting a more robust cellular response. Conclusion: This study has illuminated the protective role of hBMSC CM in mitigating the damaging effects of H2O2 on endothelial cell function. Our data demonstrate that hBMSC CM enhances the viability, proliferation, and migration of HUVECs even under oxidative stress conditions. Additionally, the conditioned medium was found to modulate the gene expression of pivotal markers related to inflammation, suggesting a favorable influence on cellular response mechanisms.
期刊介绍:
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.