Prostaglandin E2 is an unreliable biomarker for inflammation in castrated piglets: a randomized controlled trial assessing pharmaceutical drug efficiency.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

American journal of veterinary research Pub Date : 2024-07-22 Print Date: 2024-10-01 DOI:10.2460/ajvr.24.04.0096

Victoria R Merenda, Magdiel Lopez-Soriano, Stephanie Anderson, Pedro H E Trindade, Rubia M Tomacheuski, Martin S Leidig, Kristen Messenger, Juliana B Ferreira, Monique D Pairis-Garcia

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引用次数: 0

Abstract

Objective: To investigate the effect of intranasal (IN) flunixin meglumine (FM) and intra-inguinal (IG) lidocaine on castration inflammation using prostaglandin E2 (PGE2) concentration as a biomarker.

Methods: This randomized controlled trial was conducted in March 2022. Blood was collected at -24, 1, and 24 hours postcastration for PGE2 quantification from 195 piglets that received 1 of 8 treatments: (1) saline (1.5 mL) applied IG and IN (0.2 mL) followed by surgical castration (n = 24); (2) saline (1.5 mL) IG and IN (0.2 mL) followed by sham castration (25); (3) lidocaine (20 mg/kg or 1.5 mL) IG followed by surgical castration (24); (4) lidocaine (20 mg/kg or 1.5 mL) IG followed by sham castration (25); (5) FM (2.2 mg/kg) IN followed by surgical castration (25); (6) FM (2.2 mg/kg) IN followed by sham castration (24); (7) lidocaine (20 mg/kg or 1.5 mL) IG and FM (2.2 mg/kg) IN followed by surgical castration (24); and (8) lidocaine (20 mg/kg or 1.5 mL) IG and FM (2.2 mg/kg) IN followed by sham castration (24).

Results: Prostaglandin E2 concentrations did not increase following the castration procedure and were not an effective biomarker of castration inflammation. Piglets that received lidocaine demonstrated no difference in PGE2 levels across all time points. Piglets administered FM had lower PGE2 concentrations at 1 hour and 20 minutes postdrug administration in both the sham and castrated piglets.

Conclusions: Prostaglandin E2 was not an effective biomarker to quantify castration inflammation. Flunixin meglumine was able to reduce PGE2 concentration in piglets regardless of castration procedure, but lidocaine had no impact. Decreased PGE2 levels in FM-treated pigs are likely associated with the drug's ability to mitigate a noncastration-associated inflammatory process occurring independent of the castration procedure.

Clinical relevance: Flunixin meglumine reduced circulating PGE2 concentration in the blood, regardless of the castration procedure, indicating a potential for the drug to mitigate an inflammatory process unrelated to castration.

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前列腺素 E2 是阉割仔猪炎症的不可靠生物标志物：一项评估药物效率的随机对照试验。

目的以前列腺素E2（PGE2）浓度作为生物标记物，研究鼻内（IN）氟尼辛葡胺（FM）和腹股沟内（IG）利多卡因对阉割炎症的影响：这项随机对照试验于2022年3月进行。在阉割后 -24、1 和 24 小时采集 195 头仔猪的血液，对 PGE2 进行定量：(1) 生理盐水（1.5 mL）应用 IG 和 IN（0.2 mL），然后手术阉割（n = 24）；(2) 生理盐水（1.5 mL）应用 IG 和 IN（0.2 mL），然后假阉割（25）；(3) 利多卡因（20 mg/kg 或 1.5 mL）应用 IG，然后手术阉割（24）；(4) 利多卡因（20 mg/kg 或 1.5 mL）应用 IG，然后假阉割（25）；(5) FM（2.2 mg/kg) IN，然后手术阉割（25）；(6) FM (2.2 mg/kg) IN，然后假阉割（24）；(7) 利多卡因（20 mg/kg 或 1.5 mL）IG 和 FM (2.2 mg/kg) IN，然后手术阉割（24）；(8) 利多卡因（20 mg/kg 或 1.5 mL）IG 和 FM (2.2 mg/kg) IN，然后假阉割（24）：结果：阉割后前列腺素 E2 的浓度没有增加，也不是阉割炎症的有效生物标志物。接受利多卡因治疗的仔猪在所有时间点的 PGE2 水平均无差异。假阉割仔猪和阉割仔猪在用药后 1 小时和 20 分钟内的 PGE2 浓度都较低：前列腺素 E2 并非量化阉割炎症的有效生物标志物。无论阉割过程如何，氟尼辛葡甲胺都能降低仔猪体内的 PGE2 浓度，但利多卡因没有影响。经氟尼辛葡甲胺治疗的猪体内 PGE2 水平降低可能与该药物能够减轻与阉割无关的炎症过程有关：临床意义：无论阉割过程如何，氟尼辛葡甲胺都能降低血液中的循环 PGE2 浓度，这表明该药物有可能缓解与阉割无关的炎症过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.