The conformational modification of myofibrillar protein by magnetic field improves its emulsification properties

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2024-07-22 DOI:10.1016/j.ijbiomac.2024.134114

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Abstract

This study investigated the effect of different magnetic field treatments (0, 3, 6, 9, 12 mT) on the structure and emulsification properties of myofibrillar protein (MP). The results showed that the emulsion stabilized by MP with 3, 6, 9 mT magnetic field treatments possessed higher emulsifying ability, storage stability and apparent viscosity, since magnetic field induced the structural unfolding of MP and exposed the hydrophobic groups (the surface hydrophobic increased from 30.10 to 43.73 μg). Meanwhile, the magnetic field treatments decreased the MP particle size from 1752.00 to 1278.67 nm, which was favorable for the diffusion and adsorption of proteins at the oil-water interface, thus improving the MP emulsification ability and stability. Furthermore, the 9 mT magnetic field-treated MP had the best ability to emulsify oil droplets with a more uniform and smaller emulsion size from 28.593 to 23.443 μm. However, high-intensity magnetic field treatment (12 mT) caused MP particles to aggregate and the hydrophobic binding sites to be buried, which was not conducive to encapsulating oil droplets.

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磁场对肌原纤维蛋白的构象修饰改善了其乳化特性。

本研究探讨了不同磁场处理（0、3、6、9、12 mT）对肌纤维蛋白（MP）结构和乳化特性的影响。结果表明，经 3、6、9 mT 磁场处理的 MP 稳定乳液具有更高的乳化能力、贮存稳定性和表观粘度，这是因为磁场诱导了 MP 结构的解折并暴露了疏水基团（表面疏水性从 30.10 μg 增加到 43.73 μg）。同时，磁场处理使 MP 的粒径从 1752.00 nm 减小到 1278.67 nm，有利于蛋白质在油水界面的扩散和吸附，从而提高了 MP 的乳化能力和稳定性。此外，经 9 mT 磁场处理的 MP 乳化油滴的能力最强，其乳液粒度更均匀、更小，从 28.593 μm 到 23.443 μm。然而，高强度磁场处理（12 mT）会导致 MP 颗粒聚集，疏水结合位点被掩埋，不利于包裹油滴。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.