A bioactive xyloglucan polysaccharide hydrogel mechanically enhanced by Pluronic F127 micelles for promoting chronic wound healing

IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yongqi Xu , Jingyin Hu , De Bi , Wei Su , Liqing Hu , Yuxi Ma , Mengxiang Zhu , Miaoben Wu , Yuye Huang , Enxing Yu , Bing Zhang , Kailei Xu , Jing Chen , Peng Wei
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引用次数: 0

Abstract

Chronic wounds represent a formidable global healthcare challenge due to the bacteria infections and uncontrollable inflammation responses, while developing wound healing materials capable of resolving these issues remains a challenge. In this study, we integrated xyloglucan (XG) with Pluronic F127 diacrylate (F127DA)to develop a composite hydrogel for wound healing, where the XG introduced anti-inflammation and anti-bacterial properties to the construct, and F127DA provides the photocurable properties essential for hydrogel formation and robust mechanical characteristics to achieve physical strength that matches tissue regeneration. The material characterizations suggested that XG/F127DA hydrogels had great biostability, blood compatibility and antibacterial effects, which was suitable to be used as a wound healing material. The in vitro analysis by culturing L929 fibroblasts on the hydrogel surface demonstrated that the inclusion of XG could promote the cellular proliferation rate, migration rate, and re-epithelialization-related marker expression, while downregulate the inflammation process. The XG/F127DA hydrogel was further used for the full-thickness skin wound healing test on mice, where the inclusion of XG significantly increased the wound closure rate through reducing the inflammation responses, and promote re-epithelialization and angiogenesis. These results indicated that XG/F127DA hydrogel has great potential to be used for wound healing in future clinical translation.

利用 Pluronic F127 胶束增强生物活性的木聚糖多糖水凝胶,促进慢性伤口愈合。
由于细菌感染和无法控制的炎症反应,慢性伤口是全球医疗保健领域面临的一项严峻挑战,而开发能够解决这些问题的伤口愈合材料仍是一项挑战。在这项研究中,我们将木聚糖(XG)与 Pluronic F127 二丙烯酸酯(F127DA)结合在一起,开发出了一种用于伤口愈合的复合水凝胶,其中 XG 为构建物带来了抗炎和抗菌特性,而 F127DA 则为水凝胶的形成提供了必要的光固化特性和强大的机械特性,从而达到与组织再生相匹配的物理强度。材料特性表明,XG/F127DA 水凝胶具有良好的生物稳定性、血液相容性和抗菌效果,适合用作伤口愈合材料。通过在水凝胶表面培养 L929 成纤维细胞进行的体外分析表明,XG 的加入能促进细胞的增殖率、迁移率和再上皮化相关标志物的表达,同时能抑制炎症过程。XG/F127DA 水凝胶还被进一步用于小鼠全厚皮肤伤口愈合试验,结果表明,加入 XG 可通过降低炎症反应显著提高伤口闭合率,并促进再上皮化和血管生成。这些结果表明,XG/F127DA 水凝胶在未来的临床转化中具有用于伤口愈合的巨大潜力。
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来源期刊
International Journal of Biological Macromolecules
International Journal of Biological Macromolecules 生物-生化与分子生物学
CiteScore
13.70
自引率
9.80%
发文量
2728
审稿时长
64 days
期刊介绍: The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.
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