Chu-Fan Yang, Thanuka Udumulla, Ruojie Sha, James W Canary
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引用次数: 0
Abstract
Programmed DNA structures and assemblies are readily accessible, but site-specific functionalization is critical to realize applications in various fields such as nanoelectronics, nanomaterials and biomedicine. Besides pre- and post-DNA synthesis conjugation strategies, on-solid support reactions offer advantages in certain circumstances. We describe on-solid support internucleotide coupling reactions, often considered undesirable, and a workaround strategy to overcome them. Palladium coupling reactions enabled on-solid support intra- and interstrand coupling between single-stranded DNAs (ss-DNAs). Dilution with a capping agent suppressed interstrand coupling, maximizing intrastrand coupling. Alternatively, interstrand coupling actually proved advantageous to provide dimeric organic/DNA conjugates that could be conveniently separated from higher oligomers, and was more favorable with longer terphenyl coupling partners.
程序化 DNA 结构和组装很容易获得,但特定位点功能化对于实现纳米电子学、纳米材料和生物医学等各个领域的应用至关重要。除了 DNA 合成前和合成后的共轭策略外,固载反应在某些情况下也具有优势。我们介绍了通常被认为不可取的固态支持核苷酸间偶联反应,以及克服这些问题的变通策略。钯偶联反应实现了单链 DNA(ss-DNA)之间的固相支持链内和链间偶联。用封端剂稀释可抑制链间偶联,最大限度地提高链内偶联。另外,事实证明,链间偶联有利于提供二聚体有机/DNA 共轭物,便于从较高的低聚物中分离出来,而且对较长的三联苯偶联伙伴更为有利。
期刊介绍:
Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.