Nanofluidic resistive pulse sensing for characterization of extracellular vesicles†

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Lab on a Chip Pub Date : 2024-07-25 DOI:10.1039/D4LC00364K

Madalena R. C. Calado, Teresa C. Lage, Daniel A. M. André, Carlos Calaza, Carlos Marques, Carolina Herrero, João Piteira, Lars Montelius, Dmitri Y. Petrovykh, Lorena Diéguez and Alar Ainla

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Abstract

This paper describes the development, design and characterization of a resistive pulse sensing (RPS) system for the analysis of size distributions of extracellular vesicles (EVs). The system is based on microfluidic chips fabricated using soft-lithography and operated in pressure-driven mode. This fabrication approach provided reproducible pore dimensions and the best performing chip design enabled, without calibration, sizing of both 252 nm and 460 nm test particles within 8% of theoretically calculated values, based on the size specifications provided by suppliers. The number concentration measurement had higher variations and without calibration provided estimates within an order of magnitude, for sample concentrations across 4 orders of magnitude. The RPS chips could also measure successfully EVs and other biological nanoparticles in purified samples from cell culture media and human serum. A compact, fast and inexpensive RPS system based on this design could be an attractive alternative to current gold-standard techniques for routine characterization of EV samples.

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用于表征细胞外囊泡的纳米流体电阻脉冲传感技术

本文介绍了用于分析细胞外囊泡 (EV) 大小分布的电阻脉冲传感 (RPS) 系统的开发、设计和表征。该系统基于使用软光刻技术制造的微流控芯片，以压力驱动模式运行。这种制造方法提供了可重复的孔隙尺寸，性能最好的芯片设计无需校准，就能根据供应商提供的尺寸规格，将 252 nm 和 460 nm 测试颗粒的大小控制在理论计算值的 8%以内。数量浓度测量的偏差较大，在不进行校准的情况下，可对 4 个数量级的样品浓度进行一个数量级以内的估算。RPS 芯片还能成功测量细胞培养基和人体血清纯化样本中的 EVs 和其他生物纳米粒子。基于这种设计的 RPS 系统结构紧凑、快速且成本低廉，可以替代目前的黄金标准技术，用于 EV 样品的常规表征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.