High-Intensity Focused Ultrasound Enhances Drug Penetration into the Human Skin in the Franz Diffusion Cell

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-23 DOI:10.2147/ccid.s457145

Seung-Won Lee, Boncheol Leo Goo

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引用次数: 0

Abstract

Purpose: High-intensity focused ultrasound (HIFU)-assisted drug delivery is a non-invasive tool to deliver drugs to targeted areas, currently used mainly for treating cancer and cardiovascular diseases. However, in terms of transdermal drug delivery, HIFU technology is still poorly understood. Accordingly, this study sought to investigate the effectiveness of HIFU on drug penetration into the skin using human skin tissues.
Methods: Gel-type drugs whose ingredient is glutathione were labelled with fluorescein isothiocyanate, in turn the drugs were allowed to penetrate to the human skin tissue in the Franz diffusion cell for 24 hours in control and HIFU treatment groups, and their fluorescence intensity was measured using a multiple microplate reader at one, two, six, and 24 hours after drug application. In addition, tissue slice analysis was performed in each tissue slice at 24 hours post-drug application. The % area, fluorescence intensity per area, and penetration depth of the drug were measured using a fluorescence microscope.
Results: The fluorescence intensity increased with time in all groups. Specifically, at 24 hours after drug application, the fluorescence intensity (a.u). of the 10-shot HIFU treatment group was significantly enhanced compared to that of the control group (p < 0.05). The tissue slice analysis demonstrated that the % area of fluorescent drug and the fluorescence intensity per area (a.u.) were all significantly increased in both HIFU treatment groups compared to the control group (p < 0.05, p < 0.001). In addition, the penetration depth (μm) also markedly rose in both HIFU treatment groups compared to the control group (p < 0.01, p < 0.05).
Conclusion: It was demonstrated for the first time that HIFU significantly facilitated topical drug penetration into the human skin, strongly implying that HIFU can be a useful option for transdermal drug delivery.

Keywords: HIFU, transdermal drug delivery, sonophoresis, fluorescence analysis

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高强度聚焦超声能增强药物在弗朗兹扩散细胞中对人体皮肤的穿透力

目的：高强度聚焦超声（HIFU）辅助给药是一种向目标区域给药的非侵入性工具，目前主要用于治疗癌症和心血管疾病。然而，在透皮给药方面，人们对 HIFU 技术的了解还很少。因此，本研究试图利用人体皮肤组织研究 HIFU 对药物渗透到皮肤的有效性：方法：用异硫氰酸荧光素标记谷胱甘肽凝胶类药物，然后让药物在弗朗兹扩散池中渗透到人体皮肤组织中，对照组和 HIFU 治疗组各 24 小时，并在用药后 1、2、6 和 24 小时使用多微孔板阅读器测量其荧光强度。此外，还在用药后 24 小时对每个组织切片进行了组织切片分析。使用荧光显微镜测量了药物的面积百分比、单位面积荧光强度和渗透深度：结果：所有组的荧光强度都随着时间的推移而增加。具体而言，在用药 24 小时后，10 次 HIFU 治疗组的荧光强度（a.u）与对照组相比显著增强（p <0.05）。组织切片分析表明，与对照组相比，HIFU 治疗组的荧光药物面积百分比和单位面积荧光强度（a.u. ）均明显增加（p < 0.05, p < 0.001）。此外，与对照组相比，HIFU 治疗组的穿透深度（μm）也明显增加（p < 0.01, p < 0.05）：首次证明了HIFU能显著促进局部药物渗透到人体皮肤，这有力地证明了HIFU可以作为透皮给药的有效选择：HIFU、透皮给药、声波电泳、荧光分析

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.