Jasmonate signaling modulates root growth by suppressing iron accumulation during ammonium stress.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Anshika Pandey, Loitongbam Lorinda Devi, Shreya Gupta, Priti Prasad, Kanupriya Agrwal, Mehar Hasan Asif, Ajay Kumar Pandey, Kaustav Bandyopadhyay, Amar Pal Singh
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Abstract

Plants adapt to changing environmental conditions by adjusting their growth physiology. Nitrate (NO3-) and ammonium (NH4+) are the major inorganic nitrogen forms for plant uptake. However, high NH4+ inhibits plant growth, and roots undergo striking changes, such as inhibition of cell expansion and division, leading to reduced root elongation. In this work, we show that high NH4+ modulates nitrogen metabolism and root developmental physiology by inhibiting iron (Fe)-dependent Jasmonate (JA) signaling and response in Arabidopsis (Arabidopsis thaliana). Transcriptomic data suggested that NH4+ availability regulates Fe and JA-responsive genes. High NH4+ levels led to enhanced root Fe accumulation, which impaired nitrogen balance and growth by suppressing JA biosynthesis and signaling response. Integrating pharmacological, physiological, and genetic experiments revealed the involvement of NH4+ and Fe-derived responses in regulating root growth and nitrogen metabolism through modulation of the JA pathway during NH4+ stress. The JA signaling transcription factor MYC2 directly bound the promoter of the NITRATE TRANSPORTER 1.1 (NRT1.1) and repressed it to optimize the NH4+/Fe-JA balance for plant adaptation during NH4+ stress. Our findings illustrate the intricate balance between nutrient and hormone-derived signaling pathways that appear essential for optimizing plant growth by adjusting physiological and metabolic responses during NH4+/Fe stress.

茉莉酸信号通过抑制铵胁迫期间的铁积累来调节根系生长。
植物通过调整生长生理机能来适应不断变化的环境条件。硝酸盐(NO3-)和铵(NH4+)是植物吸收的主要无机氮形式。然而,高 NH4+ 会抑制植物生长,根系会发生显著变化,如抑制细胞的扩张和分裂,导致根系伸长减少。在这项研究中,我们发现高 NH4+ 通过抑制拟南芥(Arabidopsis thaliana)中依赖铁(Fe)的茉莉酸(JA)信号转导和响应,调节氮代谢和根系发育生理。转录组数据表明,NH4+的可用性调节铁和JA响应基因。高水平的 NH4+ 导致根部铁积累增加,从而抑制了 JA 的生物合成和信号响应,从而损害了氮平衡和生长。综合药理学、生理学和遗传学实验发现,在NH4+胁迫期间,NH4+和铁源反应通过调节JA途径参与调节根系生长和氮代谢。JA 信号转导因子 MYC2 直接与 NITRATE TRANSPORTER 1.1(NRT1.1)的启动子结合并抑制它,从而优化 NH4+/Fe-JA 平衡,使植物在 NH4+ 胁迫期间适应。我们的研究结果表明,在 NH4+/Fe胁迫期间,养分和激素衍生信号通路之间的平衡对于通过调整生理和代谢反应优化植物生长至关重要。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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