Morpho-molecular analyses and scanning electron microscopy reveal two new species of genus Lactarius from the northwestern Himalaya, India.

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-07-23 DOI:10.1002/jemt.24661

Komal Verma, Shiny Singh, Priyanka Uniyal, Tahir Mehmood, Yash Pal Sharma

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Abstract

One of the largest subgenera of the genus Lactarius, Lactarius subg. Russularia is abundant in the forests of the Himalayas. This article proposes Lactarius crassostipes sp. nov. and Lactarius flavocorpus sp. nov. under the L. subgenus Russularia. It includes comprehensive macro- and micro-morphological descriptions, scanning electron microscopy (SEM) micrographs, drawings, and comparisons with related species. The taxa's novelty is validated using phylogenetic analyses based on nrITS. RESEARCH HIGHLIGHTS: SEM and subsequent nrITS-based molecular phylogeny revealed two novel species (L. crassostipes and L. flavocorpus) of the genus Lactarius subg. Russularia. To further characterize the size, form, and ornamentation of these novel species' basidiospores, SEM analysis is an additional tool. In L. crassostipes "crasso" means thick and "stipes" indicates stipe. In contrast to L. flavocorpus, where "flavocorpus" refers to the golden yellow fruiting body, the name alludes to its unique thick stipe. L. crassostipes sp. nov. and L. flavocorpus sp. nov have been collected from the coniferous and broad-leaved forests of Union Territory of Jammu and Kashmir, India, respectively. The diagnostic characteristics, habit, habitat, and similarities to related species are given.

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形态分子分析和扫描电子显微镜揭示了印度喜马拉雅山西北部乳牛属的两个新物种。

作为 Lactarius 属最大的亚属之一，Lactarius 亚属 Russularia 在喜马拉雅山脉的森林中生长茂盛。本文将 Lactarius crassostipes sp.其中包括全面的宏观和微观形态描述、扫描电子显微镜（SEM）显微照片、图纸以及与相关物种的比较。该分类群的新颖性通过基于 nrITS 的系统发育分析得到了验证。研究亮点：通过 SEM 和随后基于 nrITS 的分子系统进化分析，发现了 Lactarius 亚种 Russularia 属的两个新物种（L. crassostipes 和 L. flavocorpus）。为了进一步描述这些新物种基部孢子的大小、形态和装饰，扫描电镜分析是一种额外的工具。在 L. crassostipes 中，"crasso "表示粗，"stipes "表示柄。与 L. flavocorpus 不同的是，"flavocorpus "指的是金黄色的子实体，这个名称暗指其独特的粗柄。L.crassostipessp.nov.和L.flavocorpus sp.nov分别采集自印度查谟和克什米尔联邦直辖区的针叶林和阔叶林。报告给出了这两种植物的诊断特征、习性、栖息地以及与相关物种的相似性。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.