Evaluation of mtDNA common deletion in esophageal squamous cell carcinoma.

IF 0.9 4区医学 Q4 ONCOLOGY

Indian journal of cancer Pub Date : 2024-04-01 Epub Date: 2024-07-23 DOI:10.4103/ijc.ijc_324_23

Fatemeh Ghadyani, Shahrbanoo Sharif, Saeid Morovvati

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引用次数: 0

Abstract

Background: Mitochondrial defects are thought to play a role in cancer initiation and progression for a long time. Because of the absence of protective histones and an inefficiency in the DNA repair process, mitochondrial DNA is known to be prone to mutations. The deletion of 4977bp is one of the most common mutations in human cancers. This study aimed to investigate the relationship between 4977bp common deletion and Esophageal Squamous Cell Carcinoma Disease (SCC) to provide prognostic information.

Methods: By using a PCR protocol, this study identified the 4977bp deletion of mtDNA. A PCR method was used on tumor samples from 41 squamous cell carcinoma patients and blood samples from 50 healthy individuals to detect DNA.

Results: Among the 41 tumor samples (80.5%), 33 were found to have the 4977bp deletion, while none of the blood samples from healthy individuals contained it.

Conclusions: It is shown that the deletion of 4977bp of mtDNA correlates significantly with SCC in this study. A 4977bp deletion could be used as an effective cancer screening indicator and biomarker for early diagnosis and prevention of cancer.

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评估食管鳞状细胞癌中的 mtDNA 共同缺失。

背景：线粒体缺陷被认为在癌症的发生和发展中扮演重要角色由来已久。由于缺乏保护性组蛋白以及 DNA 修复过程效率低下，线粒体 DNA 很容易发生突变。4977bp 的缺失是人类癌症中最常见的突变之一。本研究旨在探讨 4977bp 常见缺失与食管鳞状细胞癌疾病（SCC）之间的关系，以提供预后信息：本研究通过 PCR 方法确定了 mtDNA 的 4977bp 缺失。对 41 例鳞状细胞癌患者的肿瘤样本和 50 例健康人的血液样本采用 PCR 方法检测 DNA：结果：在 41 份肿瘤样本（80.5%）中，发现 33 份存在 4977bp 缺失，而健康人的血液样本中没有一份存在该缺失：结论：本研究表明，mtDNA的4977bp缺失与SCC密切相关。4977bp 缺失可作为一种有效的癌症筛查指标和生物标志物，用于癌症的早期诊断和预防。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian journal of cancer Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Indian Journal of Cancer (ISSN 0019-509X), the show window of the progress of ontological sciences in India, was established in 1963. Indian Journal of Cancer is the first and only periodical serving the needs of all the specialties of oncology in India.